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| Therapeutically Effect of a Novel Truncated Transforming Growth Factor-β Type Ⅱ Receptor Protein in Rat Hepatic Fibrosis |
| MA Hong-chuang1,2, BAI He2, ZHANG Zhe2, ZHAO Bing-hai2, CAO Ya-nan2, LI Hong-zhi2, LIU Jie-ting2, ZHANG Chun-lei2, FENG Biao3, CHU Yan-hui1,2,3 |
1. Department of Biochemistry and Molecular Biology, Jiamusi University, Jiamusi 154007, China;
2. Key Laboratory of Heilongjiang for Anti-fibrosis Biotherapy, Mudanjiang Medical University, Mudanjiang 157011, China;
3. Key Laboratory of Heilongjiang for Tissue Damage and Repair, Mudanjiang Medical University, Mudanjiang 157011, China |
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Abstract Objective: Examine the anti-fibrotic effect of a novel truncated transforming growth factor-β typeⅡ receptor (tTGF-βRⅡ) in rat hepatic fibrosis model.Methods: The recombinant truncated type TGF-betaⅡ receptor proteins was obtained from the Escherichia coli BL21 strain containing recombinant truncated TGF-betaⅡ receptor vector, by cultivating, inducing, and purification. The rat model of CCL4-induced hepatic fibrosis was established to assess the effect of the tTGF-βRⅡ protein on the treatment of fibrosis. SDS-PAGE detect the purification of truncated TGF-betaⅡ receptor proteins. The mRNA expression levels of α-SMA,Col1 and Col4 were detected by real-time PCR analysis in hepatic tissue. The content of serum ALT and AST were checked with automated biochemistry analyzer. The expression of α-SMA,Col1 and Col4 protein in hepatic tissue was detected by immunofluorescence. Liver fibrosis of the rats was evaluated by three histological methods: HE staining,Masson staining and PAS staining. Results: SDS-PAGE detection results show that the purified recombinant truncated TGF-beta Ⅱ receptor proteins was obtained. Histological examination revealed that the tTGF-βRⅡ protein prevented progression of hepatic fibrosis. The tTGF-βRⅡ protein reduced activities of serum ALT and AST. The tTGF-βRⅡ protein reduced both mRNA and protein expression of α-SMA,Col1 and Col4 in the liver tissue. The results of three histopathological staining shows that the treatment group significantly reduce the degree of liver fibrosis.Conclusion: The tTGF-βRⅡ protein prevented progression of hepatic fibrosis in rat and provided a potential treatment for liver fibrosis and other fibrosis diseases.
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Received: 24 September 2014
Published: 25 December 2014
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Cite this article:
MA Hong-chuang, BAI He, ZHANG Zhe, ZHAO Bing-hai, CAO Ya-nan, LI Hong-zhi, LIU Jie-ting, ZHANG Chun-lei, FENG Biao, CHU Yan-hui. Therapeutically Effect of a Novel Truncated Transforming Growth Factor-β Type Ⅱ Receptor Protein in Rat Hepatic Fibrosis. China Biotechnology, 2014, 34(12): 16-22.
URL:
https://manu60.magtech.com.cn/biotech/10.13523/j.cb.20141203 OR https://manu60.magtech.com.cn/biotech/Y2014/V34/I12/16
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