Please wait a minute...

中国生物工程杂志

China Biotechnology
China Biotechnology  2014, Vol. 34 Issue (10): 94-100    DOI: 10.13523/j.cb.20141015
    
Status, Problems and Strategies of Transgenic Tomatoes for Oral Vaccine
XIE Wen-qi, MA San-mei, WANG Yong-fei, SUN Xiao-wu
Department of Biotechnology, Jinan University, Guangzhou 510632, China
Download: HTML   PDF(449KB) HTML
Export: BibTeX | EndNote (RIS)      

Abstract  

Plant gene engineering techniques are developing fast, offering technical support and paving the way towards the development of transgenic plants for oral vaccines. Tomato is one of the most useful transgenic acceptors. Transgenic tomatoes for oral vaccine which can be ate directly without heating are easy to administer. This largely reduce the level of damage to the foreign protein. With the demonstration of the immune protection mechanism of genetically modified plants and the completion of the international tomato genome project, new opportunities for transgenic tomatoes are emerging. However, there still are some challenges facing in the production of transgenic tomatoes for oral vaccines. These challenges include low expression rate of foreign protein, potentiality to be digested and the food safety of genetically modified plants. Status, problems and strategies of transgenic tomatoes for oral vaccines were reviewed, which may be helpful to inspire further studies.



Key wordsTransgenic tomatoes      Oral vaccines      Foreign protein      Marker genes     
Received: 07 March 2014      Published: 25 October 2014
ZTFLH:  Q789  
Cite this article:

XIE Wen-qi, MA San-mei, WANG Yong-fei, SUN Xiao-wu. Status, Problems and Strategies of Transgenic Tomatoes for Oral Vaccine. China Biotechnology, 2014, 34(10): 94-100.

URL:

https://manu60.magtech.com.cn/biotech/10.13523/j.cb.20141015     OR     https://manu60.magtech.com.cn/biotech/Y2014/V34/I10/94


[1] Arntzen C, Plotkin S, Dodet B. Plant-derived vaccines and antibodies: Potential and limitations. Vaccine, 2005, 23(15): 1753-1756.

[2] Ahmad P, Ashraf M, Younis M, et al. Role of transgenic plants in agriculture and biopharming. Biotechnology Advances, 2012, 30(3): 524-540.

[3] Guan Z J, Guo B, Huo Y L, et al. Recent advances and safety issues of transgenic plant-derived vaccines. Applied Microbiology and Biotechnology, 2013, 97(7): 2817-2840.

[4] Shen W T, Zhou P. Research on mucosal immunity and transgenic plants for oral vaccines. China Journal of Preventive Medicine, 2003, 5: 039.

[5] Wu Z P,Xu B J. Risks of the transgenic plants into weeds in the environment. Progress in Biotechnology,1999,19(1): 9-13.

[6] Mason H S, Lam D M, Arntzen C J. Expression of hepatitis B surface antigen in transgenic plants. Proceedings of the National Academy of Sciences, 1992, 89(24): 11745-11749.

[7] Li X, Li X, Wang X, et al. Expression of epitope vaccine CTB-UA against Helicobacter Pylori in transgenic tomato. Biotechnology Frontier, 2013, 2(2):12-19.

[8] Fischhoff D A, Bowdish K S, Perlak F, et al. Insect tolerant transgenic tomato plants. Nature Biotechnology, 1987, 5(8): 807-813.

[9] Tumer N E, O'Connell K M, Nelson R S, et al. Expression of alfalfa mosaic virus coat protein gene confers cross-protection in transgenic tobacco and tomato plants. The EMBO Journal, 1987, 6(5): 1181.

[10] Kunik T, Salomon R, Zamir D, et al. Transgenic tomato plants expressing the tomato yellow leaf curl virus capsid protein are resistant to the virus. Nature Biotechnology, 1994, 12(5): 500-504.

[11] Xue B, Gonsalves C, Provvidenti R, et al. Development of transgenic tomato expressing a high level of resistance to cucumber mosaic virus strains of subgroups I and II. Plant Disease, 1994, 78(11): 1038-1041.

[12] Whitham S, McCormick S, Baker B. The N gene of tobacco confers resistance to tobacco mosaic virus in transgenic tomato. Proceedings of the National Academy of Sciences, 1996, 93(16): 8776-8781.

[13] Thomzik J E, Stenzel K, Stöcker R, et al. Synthesis of a grapevine phytoalexin in transgenic tomatoes (Lycopersicon esculentum Mill.) conditions resistance against Phytophthora infestans. Physiological and Molecular Plant Pathology, 1997, 51(4): 265-278.

[14] Sandhu J S, Krasnyanski S F, Domier L L, et al. Oral immunization of mice with transgenic tomato fruit expressing respiratory syncytial virus-F protein induces a systemic immune response. Transgenic Research, 2000, 9(2): 127-135.

[15] Jani D, Meena L S, Rizwanul-Haq Q M, et al. Expression of cholera toxin B subunit in transgenic tomato plants. Transgenic Research, 2002, 11(5): 447-454.

[16] Jiang X L, He Z M, Peng Z Q, et al. Cholera toxin B protein in transgenic tomato fruit induces systemic immune response in mice. Transgenic Research, 2007, 16(2): 169-175.

[17] Shchelkunov S N, Salyaev R K, Rekoslavskaya N I, et al. The obtaining of transgenic tomato plant producing chimerical proteins TBI-HBsAg. Doklady Biochemistry and Biophysics, 2004, 396(1): 139-142.

[18] Chen H F, Chang M H, Chiang B L, et al. Oral immunization of mice using transgenic tomato fruit expressing VP1 protein from enterovirus 71. Vaccine, 2006, 24(15): 2944-2951.

[19] Pan L, Zhang Y G, Wang Y L, et al. The expression of FMDV VP1 gene in tomatoes and the induced protective immunity in guinea pigs. Acta Microbiologica Sinica, 2006, 46(5): 796-801.

[20] McGarvey P B, Hammond J, Dienelt M M, et al. Expression of the rabies virus glycoprotein in transgenic tomatoes. Nature Biotechnology, 1995, 13(12): 1484-1487.

[21] Gao Y, Ma Y, Li M, et al. Oral immunization of animals with transgenic cherry tomatillo expressing HBsAg. World Journal of Gastroenterology, 2003, 9(5): 996-1002.

[22] Zhao W W,Ling J,Yang G P. Constraction of a plant effective expression vector driving by a fruit specitic promoter for the expression of sbr. Journal of Tropical Medicine, 2005,4(5): 135-138.

[23] Hirai T, Kim Y W, Kato K, et al. Uniform accumulation of recombinant miraculin protein in transgenic tomato fruit using a fruit-ripening-specific E8 promoter. Transgenic Research, 2011, 20(6): 1285-1292.

[24] Kurokawa N, Hirai T, Takayama M, et al. An E8 promoter-HSP terminator cassette promotes the high-level accumulation of recombinant protein predominantly in transgenic tomato fruits: a case study of miraculin. Plant Cell Reports, 2013, 32(4):1-8.

[25] Jiang X L, He Z M, Peng Z Q, et al. Cholera toxin B protein in transgenic tomato fruit induces systemic immune response in mice. Transgenic Research, 2007, 16(2): 169-175.

[26] Jani D, Meena L S, Rizwan-ul-Haq Q M, et al. Expression of cholera toxin B subunit in transgenic tomato plants. Transgenic Research, 2002, 11(5): 447-454.

[27] Sharma M K, Jani D, Thungapathra M, et al. Expression of accessory colonization factor subunit A (ACFA) of Vibrio cholerae and ACFA fused to cholera toxin B subunit in transgenic tomato (Solanum lycopersicum). Journal of Biotechnology, 2008, 135(1): 22-27.

[28] Dong L, Li H L, Liu H, et al. Progress in promoting expression levels of the vaccine protein expressed in transgenic plants. Inner Mongolia Agricultural Science and Technology, 2006, 2: 27-29.

[29] Agarwal S, Singh R, Sanyal I, et al. Expression of modified gene encoding functional human α-1-antitrypsin protein in transgenic tomato plants. Transgenic Research, 2008, 17(5): 881-896.

[30] Perlak F J, Fuchs R L, Dean D A, et al. Modification of the coding sequence enhances plant expression of insect control protein genes. Proceedings of the National Academy of Sciences, 1991, 88(8): 3324-3328.

[31] Gallie D R, Sleat D E, Watts J W, et al. The 5'-leader sequence of tobacco mosaic virus RNA enhances the expression of foreign gene transcripts in vitro and in vivo. Nucleic Acids Research, 1987, 15(8): 3257-3273.

[32] Mao L Q, Guo S D. Relationship between the length of Ω sequence and 3'poly (dA) and the gene expression efficiency. Chinese Bulletin of Botany, 1998, 40(12): 1166-1168.

[33] Cao H Y, Guo S D. Expression of human lactoferrin gene in transgenic tomato plants. Acta Horticulturae Sinica, 2005, 32(6): 59-62.

[34] Ma Y,Lin S Q,Gao Y, et al. Transformation of HBsAg (hepatitis B virus surface antigen) into tomato plants. Journal of Fujian Agricultural and Forestry University(natural science edition),2002,31(2):223-227.

[35] Daniell H. Production of biopharmaceuticals and vaccines in plants via the chloroplast genome. Biotechnology Journal, 2006, 1(10): 1071-1079.

[36] Ruf S, Hermann M, Berger I J, et al. Stable genetic transformation of tomato plastids and expression of a foreign protein in fruit. Nature Biotechnology, 2001, 19(9): 870-875.

[37] Zhou F, Badillo-Corona J A, Karcher D, et al. High-level expression of human immunodeficiency virus antigens from the tobacco and tomato plastid genomes. Plant Biotechnology Journal, 2008, 6(9): 897-913.

[38] Apel W, Bock R. Enhancement of carotenoid biosynthesis in transplastomic tomatoes by induced lycopene-to-provitamin A conversion. Plant Physiology, 2009, 151: 59-66.

[39] Wurbs D, Ruf S, Bock R. Contained metabolic engineering in tomatoes by expression of carotenoid biosynthesis genes from the plastid genome. The Plant Journal, 2007, 49: 276-288.

[40] Yang L P, Jin T C, Xu H W, et al. A new agroinoculation technology for foreign gene expression in plants by means of transient expression. Hereditas, 2013, 35(1): 111-117.

[41] Tang D S, Xia J H. Novel genetic engineering drugs. Life Science Research,1999,3(2): 92-95.

[42] Zhen Q, Guo S Q, Ge C L, et al. Review about the development of edible transgenic tomato vaccine. Chinese Medicinal Biotechnology, 2010,5(1): 57-60.

[43] Liu G F,Dong W Q,Chen L S, et al. Identification and expression of the HBV particle antigen in transgenic tomato plants. Journal of Tropical Medicine,2004,4(3): 234-236.

[44] Fuchs R L, Ream J E, Hammond B G, et al. Safety assessment of the neomycin phosphotransferase II (NPTII) protein. Biotechnology, 1993,11:1543-1547.

[45] Miki B, McHugh S. Selectable marker genes in transgenic plants: applications, alternatives and biosafety. Journal of Biotechnology, 2004, 107(3): 193-232.

[46] Kai G Y, Zhang L, Zhang H Y, et al. Marker-free: a novel tendency of transgenic plants. Acta Botanica Sinica,2002,44(8): 883-888.

[47] Dale P J, Clarke B, Fontes E M G. Potential for the environmental impact of transgenic crops. Nature Biotechnology, 2002, 20(6): 567-574.

[48] Fu Y Y, Yang Y W, Deng W, et al. Safe marker gene and its application in plant transformation. Biotechnology Bulletin,2008 (6):24-29.

[49] Li W F, Ji J, Wang G, et al. Strategies on the safety of selectable marker genes in transgenic plant. Scientia Agricultura Sinica, 2010,43(9): 1761-1770.

[50] Haldrup A, Petersen S G, Okkels F T. The xylose isomerase gene from Thermoanaerobacterium thermosulfurogenes allows effective selection of transgenic plant cells using D-xylose as the selection agent. Plant Molecular Biology, 1998, 37(2): 287-296.

[51] Peng S Q, Chen S C. A novel selection system for tomato transformation by mannose. Journal of Agricultural Biotechnology, 2005, 13(2): 141-144.

[52] Wang H W. Mannose screening system in tomato transformation. Harbin:Northeast Normal University,2009.

[53] Goldsbrough A P, Lastrella C N, Yoder J I. Transposition mediated re-positioning and subsequent elimination of marker genes from transgenic tomato. Nature Biotechnology, 1993, 11(11): 1286-1292.

[54] Zhang Y Y, Li H X, Ouyang B, et al. Chemical-induced autoexcision of selectable markers in elite tomato plants transformed with a gene conferring resistance to lepidopteran insects. Biotechnology Letters, 2006, 28(16): 1247-1253.

[55] Ma B G, Duan X Y, Niu J X, et al. Expression of stilbene synthase gene in transgenic tomato using salicylic acid-inducible Cre/loxP recombination system with self-excision of selectable marker. Biotechnology Letters, 2009, 31(1): 163-169.

[56] Zhang Y, Liu H, Li B, et al. Generation of selectable marker-free transgenic tomato resistant to drought, cold and oxidative stress using the Cre/loxP DNA excision system. Transgenic Research, 2009, 18(4): 607-619.

[57] Yang F. Expression of exogenous plant sweet protein ThaumatinⅡin marker-free transgenic tomatoes. Hangzhou:Zhejiang Normal University,2010.

[58] Khan R S, Nakamura I, Mii M. Development of disease-resistant marker-free tomato by R/RS site-specific recombination. Plant Cell Reports, 2011, 30(6): 1041-1053.

[59] Singh S, Rathore M, Goyary D, et al. Induced ectopic expression of At-CBF1 in marker-free transgenic tomatoes confers enhanced chilling tolerance. Plant Cell Reports, 2011, 30(6): 1019-1028.

[60] Wang R G, Wang S P. Foreign protein statement system and the characteristics and advantages of statement exogenous protein by plants. Bulletin of Biology,2003,38(1): 11-12.

[61] Lal P, Ramachandran V, Goyal G, et al. Edible vaccines: Current status and future. Indian J Med Microbiol, 2007 25:93-102.

[62] Hiwasa-Tanase K, Hirai T, Kato K, et al. From miracle fruit to transgenic tomato: mass production of the taste-modifying protein miraculin in transgenic plants. Plant Cell Reports, 2012, 31(3): 513-525.

[63] Chen X,Lin Z P. Production of human insulin in transgenic tomato. Molecular Plant Breeding,2003,1(4): 581-582.

[64] Liang H W, Du J C, Lu H, et al. Expression of human glucagon-like peptide 1 in transgenic tomato. Genomics and Applied Biology ISTIC, 2011, 30(4):325-330.

[65] Du J, Liang H, Lu H, et al. Producing staphylokinase with transgenic tomato. Journal of Fujian Forestry Science and Technology, 2011, 1: 010.

[66] Fu H Q, Pang S F, Xue P, et al. High levels of expression of fibroblast growth factor 21 in transgenic tobacco (Nicotiana benthamiana). Applied Biochemistry and Biotechnology, 2011, 165(2): 465-475.

[1] ZHANG Dan-feng, YU Zi-qing, WU Suo-wei, RAO Li-qun, WAN Xiang-yuan. Progress of Plant as Bioreactor in Molecular Pharming[J]. China Biotechnology, 2016, 36(1): 86-94.
[2] . Advances and Characters in the Expression of Foreign Proteins in Bacillus Megaterium[J]. China Biotechnology, 2008, 28(4): 93-97.