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中国生物工程杂志

China Biotechnology
China Biotechnology  2014, Vol. 34 Issue (10): 41-48    DOI: 10.13523/j.cb.20141007
    
Effects and Possible Mechanisms of PKA on the Development of Zebrafish Pronephron
CHEN Li, CAO Ying
School of Life Science and Technology, Tongji University, Shanghai 200092, China
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Abstract  

As an intracellular kinase, protein kinase A (PKA) functions in many tissues morphogenesis, including the central nervous system, the eye and the limb, and regulates diverse cellular activities. However, its role in kidney development is still unclear. The expression pattern of zebrafish PKA was obtained by in site hybridization and frozen-section technology; Morpholino was used for gene knockdown through microinjection; Microstructure of the pronephron was checked by immuno-fluorescence. Results showed that prkaa1 and prkab1b, coding catalytic and regulatory subunit of zebrafish PKA respectively, were maternal genes and were specifically expressed in the renal tubule epithelial cells; Inhibition of prkaa1 or prkab1b resulted in high proportion of heart edema (54.4% and 77.3% respectively) and body curvature (48.5% and 72.6% respectively) and very low proportion of polycystic kidney disease(less than 5%); Inhibition of PKA prevented migration of the renal tubule epithelial cells, especially multi-ciliated cells, resulting in prominent dilation of pronephric duct and then embryonic death. This indicated that PKA may function in zebrafish kidney development by regulating the migration of renal tubule epithelial cells.



Key wordsPKA      Cell migration      Kidney development      PKD      Zebrafish     
Received: 18 August 2014      Published: 25 October 2014
ZTFLH:  Q291  
Cite this article:

CHEN Li, CAO Ying. Effects and Possible Mechanisms of PKA on the Development of Zebrafish Pronephron. China Biotechnology, 2014, 34(10): 41-48.

URL:

https://manu60.magtech.com.cn/biotech/10.13523/j.cb.20141007     OR     https://manu60.magtech.com.cn/biotech/Y2014/V34/I10/41


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