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中国生物工程杂志

China Biotechnology
China Biotechnology
China Biotechnology  2014, Vol. 34 Issue (10): 28-34    DOI: 10.13523/j.cb.20141005
    
Culture and Characterization of a Primary Mammary Epithelial Cell line of Lysozyme Transgenic Goat
ZHU Li1,2, WANG Xue-bin2, SHI Hao3, YU Hui-qing2, LU Ping2, XU Xu-jun2, CHENG Guo-xiang2
1. School of Life Sciences Fudan University, Shanghai 200433, China;
2. Shanghai Transgenic Research Center, Shanghai 201210, China;
3. School of Animal Medicine Nanjing Agricultural University, Nanjing 210095, China
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Abstract  

The Culture and characterization of lysozyme transgenic goat mammary epithelial cell(TGMEC) were focused. The primary cell were isolated by using tissue culture method, and then purified by differential trypsin digestion. When cultured in the presence of insulin, hydrocortisone and prolactin, its growth curves comply with a typical S-type, and the chromosome number is normal when analyzed by Giemsa staining. The positive immunostaining to cytokeratin18 suggested that the resulting cell line exhibited the specific character of epithelial cells, and RT-PCR analysis showed the cell expressed endogenous milk protein (casein and lactoglobulin); Furthermore, the ELISA analysis demonstrated that this cell line also secreted recombinant lysozyme stably into the culture medium. The goat mammary epithelial cell line obtained provides an important cell model for mammary gland bioreactor studies.

Key words： Lysozyme transgenic goat      Mammary epithelial cell      Primary culture      Characterization     
Received: 01 July 2014      Published: 25 October 2014
ZTFLH:  Q819  
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Cite this article:

ZHU Li, WANG Xue-bin, SHI Hao, YU Hui-qing, LU Ping, XU Xu-jun, CHENG Guo-xiang. Culture and Characterization of a Primary Mammary Epithelial Cell line of Lysozyme Transgenic Goat. China Biotechnology, 2014, 34(10): 28-34.

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https://manu60.magtech.com.cn/biotech/10.13523/j.cb.20141005     OR     https://manu60.magtech.com.cn/biotech/Y2014/V34/I10/28


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