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中国生物工程杂志

China Biotechnology
China Biotechnology
China Biotechnology  2014, Vol. 34 Issue (9): 9-15    DOI: 10.13523/j.cb.20140903
    
Shh Signaling is Involved in Regulating BMP9-induced Osteogenic Differentiation of Mesenchymal Stem Cells
LI Li, MENG Qiu-rong, GUO Qi, WANG Lan, SHANG Lei, OU Xin-ying, LUO Jin-yong
Key Laboratory of Laboratory Medical Diagnostics, Ministry of Education, Chongqing Medical University, Chongqing 400016, China
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Abstract  

Objective: To analysis the effect of Sonic hedgehog signaling on BMP9-induced osteogenic differentiation of mesenchymal stem cells and the mechanism involved. Methods: C3H10T1/2 and C2C12 cells were treated with Cyclopamine, Purmorphamine, adenovirus Shh or/and BMP9, then the early osteogenic marker ALP activity was detected by quantitative and staining assay, the later osteogenic marker calcium deposition was detected by Alizarin Red S staining, the expressions of Shh signaling related genes and osteogenesis related genes were detected by RT-PCR, the expressions of Shh detected by Western blot, luciferae reporter assay was detected by quantitative. Results: BMP9 can promote the expressions of Shh signaling related genes. Purmorphamine and adenovirus Shh can increase the ealy and later osteogenic differentiation and expressions of osteogenesis key tromscription factors of C3H10T1/2 and C2C12 cells induced by BMP9, but Cyclopamine has the opposite effects. Furthermore, Purmorphamine and adenovirus Shh also led to luciferase activity of canonical Smad pathway stimulated by BMP9. Conclusion: Shh signaling influence BMP9-induced osteogenic differentiation of C3H10T1/2 and C2C12 mesenchymal stem cells.

Key words： Sonic hedgehog      BMP9 Mesenchymal stem cells      Oteogenic differentiation     
Received: 10 July 2014      Published: 25 September 2014
ZTFLH:  Q254  
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Cite this article:

LI Li, MENG Qiu-rong, GUO Qi, WANG Lan, SHANG Lei, OU Xin-ying, LUO Jin-yong. Shh Signaling is Involved in Regulating BMP9-induced Osteogenic Differentiation of Mesenchymal Stem Cells. China Biotechnology, 2014, 34(9): 9-15.

URL:

https://manu60.magtech.com.cn/biotech/10.13523/j.cb.20140903     OR     https://manu60.magtech.com.cn/biotech/Y2014/V34/I9/9


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