Targeted Modification of Genomic DNA by TALEs

doi:10.13523/j.cb.20140812

China Biotechnology

2014, Vol. 34

Issue (8): 74-80 DOI: 10.13523/j.cb.20140812

Targeted Modification of Genomic DNA by TALEs

ZHUANG Jun, WU Zu-jian

Fujian Provincial Key Laboratory of Plant Virology, Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou 350002, China

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Abstract

TAL (transcription activator-like) effectors secreted by plant pathogenic bacteria of genus Xanthomonasis capable of binding to specific genomic dsDNA. TAL is composed of modular architectures of DNA binding domains encompassing a tandem array of several almost identical repeat sequences. dTALEs (designed TALEs) restructured with TAL effector and other nucleotide acid-binding domains (such as nuclease, activator and suppressor) can specifically target and modify specific genomic DNA sequence, and play a pivotal role in genetic engineering. The repeat variable diresidues (RVDs) in each TAL repeat is exclusively responsible for recognition of single DNA base. TALENs consisting of TAL and restriction enzyme Fok I can contact with the specifical genomic DNA site and undergo the cleavage of dsDNA. The resulting double-stranded breaks usually are remedied through HR (homologous recombination) and NHEJ (non homologous end joining) and elicit corresponding gene mutations. TALENs are capable of generating highly efficient mutation in many model organisms. In virtue of modular design of the DNA-binding domain from TALEN system, TALENs can be developed into high-throughput platforms for gene modification and regulation and have broad-spectrum applications. Herein, an overview of the state-of-the-art structural advance of the TAL effectors, the design strategy for TALENs and applications and perspectives of TAL effector in genome-targeting modification and so on were provided.

Key words： TAL effector TALE nucleases Repeat variable diresidues Targeted mutation

Received: 04 May 2014 Published: 25 August 2014

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Cite this article:

ZHUANG Jun, WU Zu-jian. Targeted Modification of Genomic DNA by TALEs. China Biotechnology, 2014, 34(8): 74-80.

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https://manu60.magtech.com.cn/biotech/10.13523/j.cb.20140812 OR https://manu60.magtech.com.cn/biotech/Y2014/V34/I8/74

[1] Urnov F D, Rebar E J, Holmes M C, et al. Genome editing with engineered zinc finger nucleases. Nat Rev Genet, 2010, 11(9): 636-646.

[2] Carroll D. Genome engineering with zinc-finger nucleases. Genetics, 2011, 188: 773-782.

[3] Kay S, Hahn S, Marois E, et al. Bacterial effector acts as a plant transcription factor and induces a cell size regulator. Science, 2007, 318: 648-651.

[4] Römer P, Hahn S, Jordan T, et al. Plant pathogen recognition mediated by promoter activation of the pepper Bs3 resistance gene. Science, 2007, 318: 645-648.

[5] Bogdanove A J, Schornack S, Lahaye T. TAL effectors: finding plant genes for disease and defense. Curr Opin Plant Biol, 2010, 13: 394-401.

[6] Boch J, Bonas U. Xanthomonas AvrBs3 family-type Ⅲ effectors: discovery and function. Annu Rev Phytopathol, 2010, 48: 419-436.

[7] Boch J, Scholze H, Schornack S, et al. Breaking the code of DNA binding specificity of TAL-type Ⅲ effectors. Science, 2009, 326: 1509-1512.

[8] Deng D, Yan C, Pan X, et al. Structural basis of sequence-specific recognition of DNA by TAL effectors. Science, 2012, 335: 720-723.

[9] Mak A N, Bradley P, Cernadas R A, et al.The crystal structure of TAL effector PthXo1 bound to its DNA target. Science, 2012, 335:716-720.

[10] Miller J C, Tan S, Qiao G, et al. A TALE nuclease architecture for efficient genome editing. Nat Biotechnol, 2011, 29: 143-148.

[11] Morbitzer R, Römer P, Boch J, et al. Regulation of selected genome loci using de novo-engineered transcription activator-like effector (TALE)-type transcription factors. Proc Natl Acad Sci USA, 2010, 107: 21617-21622.

[12] Streubel J, Blücher C, Landgraf A, et al. TAL effector RVD specificities and efficiencies. Nat Biotechnol, 2012, 30: 593-595.

[13] Boch J, Bonas U. Xanthomonas AvrBs3 family-type Ⅲ effectors: discovery and function. Annu Rev Phytopathol, 2010, 48: 419436.

[14] .Deng D, Yan C, Wu J, et al. Revisiting the TALE repeat. Protein Cell, 2014, 5(4):297-306.

[15] Römer P, Recht S, Lahaye T. A single plant resistance gene promoter engineered to recognize multiple TAL effectors from disparate pathogens. Proc Natl Acad Sci USA, 2009, 106: 20526-20531.

[16] Römer P, Recht S, Strauss T, et al. Promoter elements of rice susceptibility genes are bound and activated by specific TAL effectors from the bacterial blight pathogen, Xanthomonas oryzae pv. oryzae. New Phytol, 2010, 187: 1048-1057.

[17] Antony G, Zhou J, Huang S, et al. Rice xa13 recessive resistance to bacterial blight is defeated by induction of the disease susceptibility gene Os-11N3. Plant Cell, 2010, 22: 3864-3876.

[18] Li T, Huang S, Zhao X, et al. Modularly assembled designer TAL effector nucleases for targeted gene knockout and gene replacement in eukaryotes. Nucleic Acids Res, 2011, 39: 6315-6325.

[19] Christian M, Cermak T, Doyle E L, et al. Targeting DNA double-strand breaks with TAL effector nucleases. Genetics, 2010, 186: 757-761.

[20] Morbitzer R, Elsaesser J, Hausner J, et al. Assembly of custom TALE-type DNA binding domains by modular cloning. Nucleic Acids Res, 2011, 39: 5790-5799.

[21] Mahfouz M M, Li L, Shamimuzzaman M, et al. De novo-engineered transcription activator-like effector (TALE) hybrid nuclease with novel DNA binding specificity creates double-strand breaks. Proc Natl Acad Sci USA, 2011, 108: 2623-2628.

[22] Mussolino C, Cathomen T. TALE nucleases: tailored genome engineering made easy. Curr Opin Biotech, 2012, 23:644-650.

[23] Cermak T, Doyle E L, Christian M, et al. Efficient design and assembly of custom TALEN and other TAL effector-based constructs for DNA targeting. Nucleic Acids Res, 2011, 39: e82.

[24] Wood A J, Lo T W, Zeitler B, et al. Targeted genome editing across species using ZFNs and TALENs. Science, 2011, 333:6040.

[25] Liu J, Li C, Yu Z, et al. Efficient and specific modifications of the Drosophila genome by means of an easy TALEN strategy. J Gen and Genom 2012, 39:209-215.

[26] Huang P, Xiao A, Zhou M, et al. Heritable gene targeting in zebrafish using customized TALENs. Nat Biotechnol, 2011, 29: 699-700.

[27] Cade L, Reyon D, Hwang W Y, et al. Highly efficient generation of heritable zebrafish gene mutations using homo-and heterodimeric TALENs. Nucleic Acids Res, 2012, 40: 8001-8010.

[28] Tong C, Huang G, Ashton C, et al. Rapid and cost-effective gene targeting in rat embryonic stem cells by TALENs. J Gen and Genom, 2012, 39:275-280.

[29] Tesson L, Usal C, Ménoret S, et al. Knockout rats generated by embryo microinjection of TALENs. Nat Biotechnol, 2011, 29: 695-696.

[30] Hockemeyer D, Wang H, Kiani S, et al. Genetic engineering of human pluripotent cells using TALE nucleases. Nat Biotechnol, 2011, 29: 731-734.

[31] Nga-Sze Mak A, Bradley P, Bogdanove A J, et al. TAL effectors: function, structure, engineering and applications. Curr Opin Struc Biol, 2012, 23:17.

[32] Mussolino C, Morbitzer R, Lütge F, et al. A novel TALE nuclease scaffold enables high genome editing activity in combination with low toxicity. Nucleic Acids Res, 2011, 39: 9283-9293.

[33] Händel E M, Alwin S, Cathomen T. Expanding or restricting the target site repertoire of zinc-finger nucleases: the inter-domain linker as a major determinant of target site selectivity. Mol Ther, 2008, 17: 104-111.

[34] Zhang F, Cong L, Lodato S, et al. Efficient construction of sequence-specific TAL effectors for modulating mammalian transcription. Nat Biotechnol, 2011, 29: 149-153.

[35] Moore F E, Reyon D, Sander J D, et al. Improved somatic mutagenesis in zebrafish using transcription activator-like effector nucleases (TALENs). PLoS ONE, 2011.6: e19509.

[36] Konermann S, Brigham M D, Trevino A E, et al. Optical control of mammalian endogenous transcription and epigenetic states. Nature. 2013, 500(7463):472-476.

[37] Deng D, Yin P, Yan C, et al. Recognition of methylated DNA by TAL effectors. Cell Research, 2012, 22:1502-1504.

[38] Engler C, Gruetzner R, Kandzia R, et al. Golden gate shuffling: a one-pot DNA shuffling method based on type Ⅱs restriction enzymes. PLoS ONE, 2009, 4: e5553.

[39] Engler C, Kandzia R, Marillonnet S. A one pot, one step, precision cloning method with high throughput capability. PLoS ONE, 2008, 3: e3647.

[40] Li T, Liu B, Spalding M H, et al. High-efficiency TALEN-based gene editing produces disease-resistant rice. Nat Biotechnol, 2012, 30: 390-392.

[41] Vainstein A, Marton I, Zuker A, et al. Permanent genome modifications in plant cells by transient viral vectors. Trends Biotechnol, 2011, 29: 363-369.

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