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中国生物工程杂志

China Biotechnology
China Biotechnology  2014, Vol. 34 Issue (4): 46-52    DOI: 10.13523/j.cb.20140408
    
Model Construction for Butanol Fermentations with Acetate/Butyrate Added by Graph Theory
LI Zhi-gang, LI Xin, SHI Zhong-ping
The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China
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Abstract  

Acetate/butyrate metabolic pathways play an important role in ABE fermentation and their changes will influence entire carbon fluxes distribution. Several fermentations with intermittent feeding of acetate/butyrate were conducted in a 7 L fermentor and the results indicated that exogenous acetate/butyrate enhanced solvents productivities by 47.1% and 39.2% respectively, and butanol/acetone ratios were improved by 21.7% for butyrate added batch and decreased by 16.2% for acetate added batch. A nonlinear constraint was utilized for acids rates calculation and the results revealed that acetate/butyrate formation pathways were almost blocked by corresponding acids feeding. Then a metabolic model constructed by graph theory was utilized to dispose those cases with acetate/butyrate added and to predict solvents production, in which some improvements were adopted based on the calculation results and related references. The model predicted solvents production, butanol/acetone ratios accurately and constructed the directed signal flow diagrams of ABE network under different conditions correctly.



Key wordsButanol fermentation      Model construction      Graph theory      Solvents productivities      Butanol/acetone ratio     
Received: 03 January 2014      Published: 25 April 2014
ZTFLH:  Q935  
Cite this article:

LI Zhi-gang, LI Xin, SHI Zhong-ping. Model Construction for Butanol Fermentations with Acetate/Butyrate Added by Graph Theory. China Biotechnology, 2014, 34(4): 46-52.

URL:

https://manu60.magtech.com.cn/biotech/10.13523/j.cb.20140408     OR     https://manu60.magtech.com.cn/biotech/Y2014/V34/I4/46


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