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| The Effects of Glutamine and Asparagine on CHO Cell Growth, Metabolism and Antibody Production |
| ZHANG Wei-yan, LIU Ya-ya, LIU Xu-ping, FAN Li, TAN Wen-song |
| State Key Lab of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China |
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Abstract The effects of glutamine and asparagine on CHO cell growth, metabolism and antibody expression in batch culture with cells being centrifuged were investigated. It was found that glutamine can't be replaced by asparagine simply, CHO-dhfr cells can't grow normally in adding separately experiments. When glutamine and asparagine concentration reached 4mmol/L meanwhile can support normal growth of CHO cells. As the total amount of glutamine and asparagine increased (0 to 24mmol/L), ammonia concentration increased linearly, however, ammonia production had no relationship with proportion of glutamine and asparagine. Furthermore, improving the asparagine and glutamine proportion moderately not only can increase the antibody production but also decrease the lactate production. In brief, the foundation of supplement of glutamine and asparagine in development and optimization of mediums and efficient fed-batch process for antibody production was established.
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Received: 10 January 2014
Published: 25 April 2014
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