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| Interruption of BMSCs into Nerve Cells Differentiation of Malignant Change of Research |
| WANG Ming-gang1, HUANG Xiao-juan1, LIAO Shi-qi2, ZHANG Hong2, WANG Jiong-rong1, MA Jin1, YANG Nan2, MA Ning2 |
1. College of life science and Engineering, Lanzhou University of Technology, Lanzhou 730050, China;
2. Gansu Academy of Medical Sciences, Lanzhou 730050, China |
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Abstract Purpose:To explore the relationship of tumor and stem cell, we report that the cells malignant transformation through interrupt normal stem cells differentiation process. Methods: Extract rat bone marrow mesenchymal stem cells (BMSCs) by whole bone marrow adherent culture method, between the induced BMSCs differentiation into neural cells, and interrupted after joining induction for diffent times. On the fourth normal culture generation, through the cell morphological observation, immunohistochemical detection, cellular pathology identification, soft AGAR integrated experiment and CTX immunosuppressive mice renal fascia vaccination experiments show that obvious malignant transformation. Results: The cells induced difftent times cells have characteristics of the nerve cells, but not completely differentiation; Cell morphological pathology identification performance for cell contact inhibition disappears, the nucleus mass ratio, soft AGAR integration form experiments and animal experiments show that 40 h interrupt cells undergo malignant transformation and owe cloned formation ability and tumorigenicity. Conclusion: The cultivation of the interrupt normal differentiation of BMSCs cell has a tendency to into tumor cells.
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Received: 03 December 2013
Published: 25 March 2014
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