Cloning and Analysis of the Gene Encoding Cinnamoyl-CoA Reductase from <em>Caragana korshinkii</em> Kom

doi:10.13523/j.cb.20140108

China Biotechnology

2014, Vol. 34

Issue (1): 50-56 DOI: 10.13523/j.cb.20140108

Cloning and Analysis of the Gene Encoding Cinnamoyl-CoA Reductase from Caragana korshinkii Kom

LI Gao^1,2, YANG Qi¹, ZHANG Ye^1,3, WANG Yin¹, ZHANG Tao¹, WANG Rui-gang¹, LI Guo-jing¹

1. College of Life Sciences, Inner Mongolia Agricultural University, Hohhot 010018, China;
2. Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China;
3. The Ordos Food and Drug Inspection and Research Center, Ordos 017000, China

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Abstract Cinnamoyl-CoA reductase (CCR, EC 1.2.1.44) plays an important role in the phenylpropanoid pathway. A gene coding for CCR was isolated from Caragana korshinkii Kom. by rapid amplification of cDNA ends technique, designated as CkCCR (GenBank accession no. HQ829859). Full-length cDNA is 1 270 bp in length with a 1 014bp open reading frame. The protein deduced from cDNA is 434 amino acids with a calculated molecular weight of 48kDa and an isoelectric point of 6.42. Sequence alignments revealed that CkCCR had close similarity with CCRs from other species,it contains NADPH binding sequence and a conserved region "KNWYCYGKA". Phylogenetic analysis indicated that the CkCCR stayed at the same evolutionary branch with the AtCCR2 and shared the closest homology with the CCR from Leucaena glauca. The results of real-time-quantitative analysis showed that the transcripts of CkCCR expressed constitutively in root, stem and leaves. In addition, the expression levels of CkCCR decreased after drought treatment at the beginning, then returned to the expression level of the control at late time points under drought condition.

Key words： Caragana korshinskii Kom. CCR RACE Sequence analysis

Received: 22 January 2013 Published: 25 January 2014

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LI Gao, YANG Qi, ZHANG Ye, WANG Yin, ZHANG Tao, WANG Rui-gang, LI Guo-jing. Cloning and Analysis of the Gene Encoding Cinnamoyl-CoA Reductase from Caragana korshinkii Kom. China Biotechnology, 2014, 34(1): 50-56.

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https://manu60.magtech.com.cn/biotech/10.13523/j.cb.20140108 OR https://manu60.magtech.com.cn/biotech/Y2014/V34/I1/50

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