| Orginal Article |
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| The Physiological Mechanism for Enhanced Cyclic Adenosine Monophosphate Biosynthesis by Auxiliary Energy Substance |
LI Zhi-gang1,2,CHEN Bao-feng2,ZHANG Zhong-hua2,CHANG Jing-ling1,2,**( ) |
1 Collaborative Innovation Center of Modem Biological Breeding of Henan Province, Xinxiang 453003,China
2 School of Life Science and Technology, Henan Institute of Science and Technology, Xinxiang 453003,China |
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Abstract High ATP level is beneficial to cyclic adenosine phosphate (cAMP) accumulation. Due to 3g/L-broth sodium pyruvate addition into the broth at 24h, cAMP final content achieved 4.13g/L which was enhanced 24.4% when compared with control, with obvious improved fermentation performance. NADH, ATP concentrations and activities of key enzymes contained in related metabolic pathways were detected to explain the mechanism. The results declare that pyruvate kinase was inhibited obviously by sodium pyruvate and the activities of key enzymes for cAMP synthesis (glucose-6-phosphate dehydrogenase, adenylosuccinate synthetase and adenylate cyclase) were enhanced greatly, which indicated that more carbon flux was directed into pentose phosphate pathway and purine synthesis pathway from glycolytic pathway for AMP production. In addition, activities of key enzymes in TCA (isocitrate dehydrogenase and succinate dehydrogenase), NADH/NAD +, ATP/AMP and respiratory chain activity were enhanced greatly which indicated that pyruvate activated energy metabolism to supply sufficient energy for cAMP synthesis. Sufficient energy supplement and enhanced carbon flux accounts for the physiological mechanism of higher cAMP accelerated by sodium pyruvate.
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Received: 12 May 2019
Published: 27 March 2020
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Corresponding Authors:
Jing-ling CHANG
E-mail: changjl001@126.com
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