免疫信息学在表位疫苗研发中的应用与研究进展<sup>*</sup>

doi:10.13523/j.cb.2301022

中国生物工程杂志

2023, Vol. 43

Issue (7): 88-100 DOI: 10.13523/j.cb.2301022

综述

免疫信息学在表位疫苗研发中的应用与研究进展^*

冯宏盛^1,²,金行^1,²,高永宇³,鲜钰涵^1,²,李海洋^1,²,杨思宇^1,²,贾爱明^4,^**(

),高凤山^1,^2,^**(

)

1 大连大学生命健康学院大连 116622
2 大连市基因和蛋白质工程药物筛选及研发重点实验室大连 116622);(3 吉林农业大学动物医学院长春 130118
3 大连医科大学附属第二医院大连 116027

Application of Immunoinformatics in Epitope Vaccine Development

Hong-sheng FENG^1,²,Hang JIN^1,²,Yong-yu GAO³,Yu-han XIAN^1,²,Hai-yang LI^1,²,Si-yu YANG^1,²,Ai-ming JIA^4,^**(

),Feng-shan GAO^1,^2,^**(

)

1 College of Life and Health, Dalian University, Dalian 116622, China
2 The Dalian Gene and Protein Engineering for Drug Screening Key Laboratory, Dalian 116622, China
3 College of Veterinary Medicine, Jilin Agricultural University, Changchun 130118, China
4 The Second Hospital of Dalian Medical University, Dalian 116027, China

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摘要：

对于表位疫苗的设计,通常是利用免疫信息学工具和相关技术方法对已获取或已知的核苷酸序列和氨基酸序列进行分析,以此确定和预筛选出可能的优势表位,之后通过人工合成或基因工程技术等制备具有优势表位的多肽疫苗。免疫信息学的快速发展使其被成功应用于疫苗学领域,成为开发基于表位多肽疫苗最有效的方法。从免疫信息学在表位疫苗设计中的一般流程及疫苗验证、表位疫苗研发涉及的免疫信息学工具以及免疫信息学在表位疫苗设计中的具体应用等方面进行综述,以期为合理设计和研发有效的表位疫苗提供参考。

关键词： 免疫信息学; 表位多肽; 表位疫苗

Abstract:

Presently, to design epitope vaccines, the research and development process is generally to use computer-aided immunoinformatics tools and related technical methods to analyze acquired or known nucleotide and amino acid sequences to determine and pre-screen out possible dominant epitopes, and then prepare polypeptide vaccines with dominant epitopes through synthetic or genetic engineering techniques. The rapid development of immunoinformatics has been successfully applied to the field of vaccinology, and the immunoinformatics method is the most effective method to develop vaccines based on epitope polypeptide. Immunoinformatics, the general process of immunoinformatics in epitope vaccine design and validation, the immunoinformatics tools involved in the design of epitope vaccines, and the specific application of immunoinformatics in the design of epitope vaccines are reviewed, which will provide reference for reasonable design and development of effective epitope vaccines.

Key words: Immunoinformatics Epitope peptides Epitope vaccines

收稿日期: 2023-01-15 出版日期: 2023-08-03

ZTFLH:

Q816

基金资助: 国家自然科学基金(32273022)

通讯作者: **电子信箱:jiaam200310@163.com;gfsh0626@126.com

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	冯宏盛
	金行
	高永宇
	鲜钰涵
	李海洋
	杨思宇
	贾爱明
	高凤山

引用本文:

冯宏盛, 金行, 高永宇, 鲜钰涵, 李海洋, 杨思宇, 贾爱明, 高凤山. 免疫信息学在表位疫苗研发中的应用与研究进展^*[J]. 中国生物工程杂志, 2023, 43(7): 88-100.

Hong-sheng FENG, Hang JIN, Yong-yu GAO, Yu-han XIAN, Hai-yang LI, Si-yu YANG, Ai-ming JIA, Feng-shan GAO. Application of Immunoinformatics in Epitope Vaccine Development. China Biotechnology, 2023, 43(7): 88-100.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/10.13523/j.cb.2301022 或 https://manu60.magtech.com.cn/biotech/CN/Y2023/V43/I7/88

图1 利用免疫信息学设计表位疫苗的基本流程

表1 疫苗研发后常用的验证方法

表2 T细胞表位预测的部分在线使用工具

表3 B细胞表位预测的部分在线使用工具

表4 表位分析的部分在线使用工具

表5 疫苗构建体的结构预测在线使用工具

表6 分子对接在线使用工具

图2 针对DENV的多表位肽疫苗的设计流程

图3 针对布鲁氏菌的多表位候选疫苗的设计流程

图4 针对弓形虫感染的多表位候选疫苗的设计流程

图5 新型PD-1 B细胞肽表位疫苗的设计流程

图6 基于Amb a 11的多表位疫苗设计流程

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