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中国生物工程杂志

CHINA BIOTECHNOLOGY
中国生物工程杂志
中国生物工程杂志  2022, Vol. 42 Issue (12): 111-119    DOI: 10.13523/j.cb.2206061
综述     
低共熔溶剂对酶的影响及应用研究*
赵楠杉1,2,赵亚鑫1,张海花1,杨东风1,2,梁宗锁1,2,郭建军1,2,**()
1 浙江理工大学生命科学与医药学院 杭州 310018
2 浙江理工大学绍兴生物医药研究院 绍兴 312090
Effects of Deep Eutectic Solvents on Enzyme
ZHAO Nan-shan1,2,ZHAO Ya-xin1,ZHANG Hai-hua1,YANG Dong-feng1,2,LIANG Zong-suo1,2,GUO Jian-jun1,2,**()
1 College of Life Sciences and Medicine, Zhejiang Sci-tech University, Hangzhou 310018, China
2 Zhejiang Sci-tech University Shaoxing Academy of Biomedicine, Shaoxing 312090, China
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摘要：

低共熔溶剂是由一定化学计量比的氢键受体和氢键供体组合而成的新型绿色溶剂,具有成本低、易制备、环境友好等特点,可以作为普通有机溶剂和离子液体的替代溶剂。酶作为生物催化剂时反应条件温和,对反应底物专一性高,并且具有极高的催化效率和反应速度。酶促反应通常发生在水溶液体系,但近年来发现在低共熔溶剂中酶促反应也能有效进行。综述酶与低共熔溶剂共同作用的机理以及低共熔溶剂在酶促反应中的应用,展望未来的研究方向,为酶促反应体系的进一步开发奠定理论基础。
关键词： 低共熔溶剂;  酶;  催化    
Abstract:

Deep eutectic solvent is a new type of green solvent, which is composed of hydrogen bond acceptor and hydrogen bond donor with a certain stoichiometric ratio. It has low preparation cost and is friendly to the environment. It can be used as an alternative solvent for ordinary organic solvents and ionic liquids.As a biocatalyst, enzyme has mild reaction conditions, high substrate specificity, and high catalytic efficiency and reaction speed. Enzymatic reaction usually occurs in aqueous solution, but recently it has been found that it can also be carried out effectively in deep eutectic solvents. In order to better understand the effects of deep eutectic solvents on enzyme, the mechanism of the interaction between enzyme and deep eutectic solvents and the latest progress in application are systematically discussed. At the same time, the advantages and disadvantages of deep eutectic solvents in enzymatic reaction are evaluated. Finally, the future development prospects are discussed.
Key words: Deep eutectic solvents    Enzyme    Catalysis
收稿日期: 2022-06-30 出版日期: 2023-01-05
ZTFLH:  Q814  
基金资助: *浙江省“尖兵”“领雁”研发攻关计划资助项目(2022C02023)
通讯作者: 郭建军     E-mail: biojjguo@zstu.edu.cn
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引用本文:

赵楠杉,赵亚鑫,张海花,杨东风,梁宗锁,郭建军. 低共熔溶剂对酶的影响及应用研究*[J]. 中国生物工程杂志, 2022, 42(12): 111-119.

ZHAO Nan-shan,ZHAO Ya-xin,ZHANG Hai-hua,YANG Dong-feng,LIANG Zong-suo,GUO Jian-jun. Effects of Deep Eutectic Solvents on Enzyme. China Biotechnology, 2022, 42(12): 111-119.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/10.13523/j.cb.2206061    或    https://manu60.magtech.com.cn/biotech/CN/Y2022/V42/I12/111

图1  常见氢键供体和氢键受体结构式
种类 示例 主要应用领域
金属盐+季铵盐 ChCl-ZnCl2 有毒气体去除[10]
水合金属盐+季铵盐 ChCl-CrCl3·6H2O 大规模工业过程[11]
氢键供体+季铵盐 ChCl-Urea 天然产物提取[12]、金属电沉积[13-14]、制备生物柴油[15]
氢键供体+金属水合盐 MgCl2·6H2O-(CH2OH)2 合成纳米结构[16]
表1  低共熔溶剂的分类及应用
原材料 低共熔溶剂 配比 辅助手段 目标产物 产量 参考文献
洋蓍草 氯化胆碱∶乳酸 1∶2 超声 总酚 (35.44 ± 2.12)mg/g [32]
爪哇龙船花 氯化胆碱∶1,2-丙二醇 1∶1 超声 阿魏酸 (12.937 ± 0.169)mg/g [33]
黄芪 氯化胆碱∶尿素 1∶2 加热 没食子酸 (1.89 ± 0.05)μg/mg [34]
鞣花酸 (12.76 ± 0.13)μg/mg
可水解单宁 (181.26 ± 0.159)μg/mg
黑胡萝卜 氯化胆碱∶柠檬酸 - 超声 花青素-3-O-糖苷 (16.855 ± 0.09)mg/g [35]
粟细糠 甜菜碱∶甘油 1∶2 超声 总酚 (7.80 ± 0.09)mg/g [36]
甜叶菊 1,2-丙二醇∶甘油∶水 8∶1∶1 超声 甜菊苷 3.48 mg/mL [37]
复方甘草片 1,4-丁二醇∶乙酰丙酸 1∶3 超声 甘草苷 5.6 mg/g [38]
异甘草苷 3.17 mg/g
甘草素 1.27 mg/g
甘草酸 74.62 mg/g
异甘草素 1.34 mg/g
文冠果 四丙基溴化铵∶乳酸 1∶2 加热 皂苷 (72.11 ± 0.61)mg/g [39]
鸡矢藤 氯化胆碱∶乙二醇 1∶2 超声 总黄酮 27.04 mg/g [26]
显齿蛇葡萄叶 氯化胆碱∶葡萄糖 4∶1 超声 黄酮 83.93% [27]
云杉叶 氯化胆碱∶二羧酸 1∶1 - 黄酮 217.56 μg/mL [12]
表2  低共熔溶剂提取植物活性成分
图2  低共熔溶剂与木质纤维类生物质的生产工艺
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