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中国生物工程杂志

CHINA BIOTECHNOLOGY
中国生物工程杂志
中国生物工程杂志  2022, Vol. 42 Issue (8): 30-39    DOI: 10.13523/j.cb.2204070
研究报告     
利用海洋微拟球藻生产蜂王浆主效成分24-亚甲基胆固醇的研究*
邓湘子,周文序,路延笃**()
海南大学海洋学院 南海海洋资源利用国家重点实验室 海口 570228
Production of 24- Methylenecholesterol from Royal Jelly by Nannocloropsis oceanica IMET1
DENG Xiang-zi,ZHOU Wen-xu,LU Yan-du**()
State Key Laboratory of Marine Resource Utilization in South China Sea, College of Oceanology, Hainan University, Haikou 570228, China
 全文: PDF(3527 KB)   HTML
摘要：

用于生产生物燃料的微藻生物技术正处于十字路口,但仍在不断发展。微藻衍生的多产品技术的发展将大大提高经济可行性,特别是结合生产高附加值化合物。研究结果表明敲除海洋微拟球藻中的DWARF1 (DWF1)基因的突变株具有比野生型更高的色素含量和光合效率,并且还可以显著降低胆固醇积累(这可能是人类心血管疾病的主要危险因素),使其从原本占总甾醇 (TSs) 超过70%的含量减少至零。相比之下,其前体24-亚甲基胆固醇(一种对人类健康有益的蜂王浆的关键微量营养素)的产量则从零增加到60%以上。结合海洋微拟球藻中ω-3脂肪酸含量高的特点,我们预计在工业规模上开发该菌株将带来可观的利润。
关键词： 微拟球藻;  DWARF1基因;  胆固醇;  24-亚甲基胆固醇;  植物甾醇;  蜜蜂人工饲料蜂王浆    
Abstract:

Microalgal biotechnology for biofuels is at a crossroads and its development is still in flux. The development of microalga-derived multi-product technology will greatly improve the economic viability, particularly with a combination of the production of high value-added compounds. The results showed that the mutants with DWARF1 (DWF1) gene knocked out had higher pigment content and photosynthetic efficiency than the wild type, and could also significantly reduce the accumulation of cholesterol (which might act as a leading risk factor for human cardiovascular disease) from over 70% of total sterols (TSs) to null. In contrast, the production of its precursor 24-methylenecholesterol (a critical micronutrient of royal jelly that is beneficial to human health) was increased from null to more than 60% of TSs. Combined with the high content of omega-3 fatty acids of N. oceanica, we anticipate an appreciable profit by exploiting this strain on an industrial scale.
Key words: Nannocloropsis oceanica    DWARF1 gene    Cholesterol    24-Methylenecholesterol    Phytosterols    Honeybee artificial food    Royal jelly
收稿日期: 2022-04-27 出版日期: 2022-09-07
ZTFLH:  Q819  
基金资助: * 国家重点研发计划合成生物学重点专项(2021YFA0909600);国家重点研发计划政府间合作重点专项(2021YFE0110100);国家自然科学基金(3120006);海南省研究生创新科研项目(QHYS2021-209)
通讯作者: 路延笃     E-mail: ydlu@hainanu.edu.cn
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引用本文:

邓湘子, 周文序, 路延笃. 利用海洋微拟球藻生产蜂王浆主效成分24-亚甲基胆固醇的研究*[J]. 中国生物工程杂志, 2022, 42(8): 30-39.

DENG Xiang-zi, ZHOU Wen-xu, LU Yan-du. Production of 24- Methylenecholesterol from Royal Jelly by Nannocloropsis oceanica IMET1. China Biotechnology, 2022, 42(8): 30-39.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/10.13523/j.cb.2204070    或    https://manu60.magtech.com.cn/biotech/CN/Y2022/V42/I8/30

图1  dwf1突变株基因组DNA的PCR扩增
图2  正常培养条件下dwf1突变株和野生型的生长表现
Sterols kinds RT/min Sterol profile/%
dwf1-1 dwf1-7 WT
Cholesterol 14.34 1.02±0.03* 1.21±0.09* 73.87±1.01
Desmosterol 15.09 17.49±0.90* 15.43±1.09* 0.00±0.00
24-Methylenecholesterol 16.39 62.15±2.26* 66.63±1.45* 0.00±0.00
Clerosterol 18.13 1.02±0.13 0.78±0.03* 1.17±0.06
Isofucosterol 18.59 8.79±0.67* 7.62±0.17* 10.75±0.29
Fucosterol 18.99 9.53±0.53* 8.32±0.25* 14.22±0.69
Total sterol/(μg/mg DW) — 4.08±0.55 5.69±0.53* 4.43±0.19
表1  正常培养条件下野生型和dwf1突变株的GC-MS 甾醇数据分析
图S1  野生型微拟球藻的基因组序列
图S2  dwf1-1突变株的基因组序列
图S3  dwf1-7突变株的基因组序列
图S4  微拟球藻中的主要甾醇
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