中国生物工程杂志

Photodynamic therapy (PDT) has the advantages of minimally invasive, controllable, low toxicity, and repeatable treatment, and has become an indispensable treatment method in clinical medicine. However, due to the self-protection mechanism of tumor cells, the efficacy of PDT is greatly reduced. This study uses PDT therapy while implementing pharmacological autophagy suppression strategies to cut off protective autophagy under severe oxidative damage due to photodynamic therapy. The porphyrin metal organic framework PCN-224 was synthesized by the oil bath heating method, and the autophagy inhibitor hydroxychloroquine sulfate (HCQ) was loaded on the PCN-224, and the scanning electron microscope (SEM), particle size test (DLS), UV visible spectral testing and other methods showed that the material was successfully synthesized, which enhanced the water solubility of the porphyrin photosensitizer, and significantly enhanced the toxicity to 4T1 mouse breast cancer cells after light, and it further improved the tumor killing ability after being loaded with HCQ. HCQ can be released in tumor cells to deacidify lysosomes and inhibit autophagy, cut off the protective autophagy under severe oxidative damage due to photodynamic therapy, and achieve the effect of killing tumors.