| 技术与方法 |
|
|
|
|
| 技术与方法体外组装含有组蛋白变体H2A.Z及H3.3的核小体 |
| 赵宏宇1,2, 刘媛2, 张凤慧2, 邢永强2, 蔡禄2 |
1. 内蒙古大学物理科学与技术学院 呼和浩特 010021;
2. 内蒙古科技大学数理与生物工程学院 包头 014010 |
|
| Assembly Nucleosome Containing Histone Variant H2A.Z and H3.3 in vitro |
| ZHAO Hong-yu1,2, LIU Yuan2, ZHANG Feng-hui2, XING Yong-qiang2, CAI Lu2 |
1. School of Physical Science and Technology, Inner Mongolia University, Hohhot 010021, China;
2. School of Mathematics Physics and Biological Engineering, Inner Mongolia University of Science and Technology, Baotou 014010, China |
引用本文:
赵宏宇, 刘媛, 张凤慧, 邢永强, 蔡禄. 技术与方法体外组装含有组蛋白变体H2A.Z及H3.3的核小体[J]. 中国生物工程杂志, 2016, 36(3): 53-60.
ZHAO Hong-yu, LIU Yuan, ZHANG Feng-hui, XING Yong-qiang, CAI Lu. Assembly Nucleosome Containing Histone Variant H2A.Z and H3.3 in vitro. China Biotechnology, 2016, 36(3): 53-60.
链接本文:
https://manu60.magtech.com.cn/biotech/CN/DOI:10.13523/j.cb.20160308
或
https://manu60.magtech.com.cn/biotech/CN/Y2016/V36/I3/53
|
| [1] Chen P, Zhao J, Wang Y, et al. H3.3 actively marks enhancers and primes gene transcription via opening higher-ordered chromatin. Genes Dev, 2013, 27(19): 2109-2124.
[2] Chen P, Wang Y, Li G. Dynamics of histone variant H3.3 and its coregulation with H2A.Z at enhancers and promoters. Nucleus, 2014, 5(1): 21-27.
[3] Suto R K, Clarkson M J, Tremethick D J, et al. Crystal structure of a nucleosome core particle containing the variant histone H2A.Z. Nat Struct Biol, 2000, 7(12): 1121-1124.
[4] Sutcliffe E L, Parish I A, He Y Q, et al. Dynamic histone variant exchange accompanies gene induction in T cells. Mol Cell Biol, 2009, 29(7): 1972-1986.
[5] Jin C, Zang C, Wei G, et al. H3.3/H2A.Z double variant-containing nucleosomes mark ‘nucleosome-free regions’ of active promoters and other regulatory regions. Nat Genet, 2009, 41(8): 941-945.
[6] Goldberg A D, Banaszynski L A, Noh K M, et al. Distinct factors control histone variant H3.3 localization at specific genomic regions. Cell, 2010, 140(5): 678-691.
[7] Trifonov E N, Nibhani R. Review fifteen years of search for strong nucleosomes. Biopolymers, 2015, 103(8): 432-437.
[8] Zhao H, Xing Y, Liu G, et al. GAA triplet-repeats cause nucleosome depletion in the human genome. Genomics, 2015, 106(2): 88-95.
[9] 蔡禄. 表观遗传学前沿. 北京:清华大学出版社,2012. Cai L. Forefront of Epigenetics. Beijing:Tsinghua University Press, 2012.
[10] 王成爱,赵宏宇,邢永强,等.酿酒酵母核小体定位理论模型的体外实验验证.生命科学研究,2014, 18(1): 21-27. Wang C, Zhao H, Xing Y, et al. In vitro experimental verification of theoretical model of nucleosome positioning on Saccharomyces cerevisiae. Life Science Research, 2014, 18(1): 21-27.
[11] Lusser A, Kadonaga J T. Strategies for the reconstitution of chromatin. Nat Methods, 2004, 1(1): 19-26.
[12] Peckham H E, Thurman R E, Fu Y, et al. Nucleosome positioning signals in genomic DNA. Genome Res, 2007, 17(8): 1170-1177.
[13] Kaplan N, Moore I K, Fondufe-Mittendorf Y, et al. The DNA-encoded nucleosome organization of a eukaryotic genome. Nature, 2009, 458(7236):362-366.
[14] Segal E, Widom J. Poly(dA:dT) tracts: major determinants of nucleosome organization. Curr Opin Struct Biol, 2009, 19(1):65-71. |
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
| |
Shared |
|
|
|
|
| |
Discussed |
|
|
|
|
