技术与方法 |
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Nodal检测双单抗夹心ELISA法建立及应用 |
吴孟玲, 周嘉旺, 杜军 |
中山大学药学院 微生物与生化药学实验室 广州 510006 |
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Development and Application of A Double Monoclonal Antibody Sandwich ELISA for the Assay of Nodal |
WU Meng-ling, ZHOU Jia-wang, DU Jun |
Department of Microbial and Biochemical Pharmacy, School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou 510006, China |
[1] Conleyba T S. Prognostic and predictive markers in cancer. DIS Markers, 2004, 20(2):35-43.
[2] Sturgeon C. Practice guidelines for tumor marker use in the clinic. Clinical Chemistry, 2002, 48(8):1151-1159.
[3] 杨立新,郁卫东,刘桂生,等.Nodal信号的研究进展.中国生物工程杂志,2003,23(3):15-19. Yang L X, Yu W D, Liu G S, et al. Recent advances in nodal signaling. China Biotechnology, 2003,23(3):15-19.
[4] Seo H J, Kim Y J, Cho K B, et al. Nodal metastasis after successful endoscopic submucosal dissection for colorectal mucosal cancer. Endoscopy, 2011, 43(Suppl 2 UCTN):E374-E375.
[5] Quail D F, Walsh L A, Zhang Gui-hua, et al. Embryonic protein nodal promotes breast cancer vascularization. Cancer Research, 2012, 72(15):3851-3863.
[6] 方瑞,杜军.Nodal信号调控与肿瘤的研究现状.中华肿瘤防治杂志,2012,19(11):872-876. Fang R, Du J. Research advances of regulation of Nodal signal and tumor. Chinese Journal of Cancer Prevention and Treatment, 2012,19(11):872-876.
[7] Strizzi L, Hardy K M, Kirschmann D A, et al. Nodal expression and detection in cancer:experience and challenges. Cancer Research, 2012, 72(8):1915-1920.
[8] Engvall E, Karin J, Perlmann P. Enzyme-linked immunosorbent assay. II. Quantitative assay of protein antigen, immunoglobulin G, by means of enzyme-labelled antigen and antibody-coated tubes. Biochimica ET Biophysica ACTA (Bba)-Protein STRU, 1971, 251(3):427-434.
[9] Zangar R C, Daly D S, White A M. ELISA microarray technology as a high-throughput system for cancer biomarker validation. Expert Review of Proteomics, 2006, 3(1):37-44.
[10] Gan S D, Patel K R. Enzyme immunoassay and enzyme-linked immunosorbent assay. Journal of Investigative Dermatology, 2013, 133(9):1-3.
[11] 李玲玲,江冠民,张革,等.人源Nodal成熟肽的原核表达及多克隆抗体的制备.细胞与分子免疫学杂志,2011,27(8):880-882,88. Li L L, Jiang G M, Zhang G, et al. Prokaryotic expression and polyclonal antibody preparation of human Nodal mature peptide. Journal of Cellular and Molecular Immunology, 2011,27(8):880-882,88.
[12] 陈万青,张思维,曾红梅,等.中国2010年恶性肿瘤发病与死亡.中国肿瘤,2014,23(1):1-10. Chen M Q, Zhang S W, Zeng H M et al. Report of cancer incidence and mortality in China,2010. Bulletin of Chinese Cancer, 2014,23(1):1-10.
[13] Lech G, Ssotw n' ski R, Sodkowski M, et al. Colorectal cancer tumour markers and biomarkers:recent therapeutic advances. World Journal of Gastroenterology, 2016,22(5):1745-1755.
[14] Goulart B L, Clark J W, Pien H H, et al. Trends in the use and role of biomarkers in phase I oncology trials. Clinical Cancer Research, 2007, 13(22):6719-6726.
[15] Hsu S M, Raine L, Fanger H. Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques a comparison between ABC and unlabeled antibody (PAP) procedures. The Journal of Histochemistry and Cytochemistry, 1981, 29(4):577-580.
[16] Grumbach I M, Veh R W. The SA rABC technique a new ABC procedure for detection of antigens at increased sensitivity. The Journal of Histochemistry and Cytochemistry, 1995, 43(1):31-37. |
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