建立金线莲原球茎悬浮体系生产次生代谢产物

中国生物工程杂志

2012, Vol. 32

Issue (05): 43-50

研究报告

建立金线莲原球茎悬浮体系生产次生代谢产物

吕志伟^1,2, 吴文平², 毕丽伟¹, 江星星², 王雅英¹

1. 厦门医学高等专科学校厦门 361008;
2. 厦门爱格瑞生物科技有限公司厦门 361100

Establishment of Protocorm-like Bodies of Anoectochilus Formosanus Hayata in Suspension Culture for the Production of Secondary Metabolites

LV Zhi-wei^1,2, WU Wen-ping², BI Li-wei¹, JIANG Xing-xing², WANG Ya-ying¹

1. Xiamen Medical College, Xiamen 361008, China;
2. Research Center of Xiamen Igreen Bio-tech Co. Ltd., Xiamen 361100, China

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摘要：

研究植物激素浓度和培养周期对金线莲原球茎悬浮培养生长及其代谢产物积累的影响,以增加金线莲悬浮培养的生长量,提高次生代谢产物的生产。结果表明,MS培养基添加S - 3307 1.0mg/L,6 - BA 0.5mg/L和3％的蔗糖适合总生物量的生长(214.45g/L,FW和18.23g/L DW)。而MS培养基添加S -3307 1.0mg/L,6 - BA 3.0mg/L和5％的蔗糖,总黄酮,总酚和多糖的干重(5.43mg/g,2.87mg/g和243.23mg/g)达到最大化。研究原球茎悬浮培养过程,发现经过7个星期培养就能获得最大的生物质总量(225.98 g/L的FW和18.53 g/L的DW)、总黄酮干重(5.09mg/g)和总酚干重(2.04mg/g),而多糖生产达到其峰值(229.36mg/g干重)是在培养后5个星期。

关键词： 金线莲; 原球茎悬浮培养; 次生代谢产物

Abstract:

The growth and secondary metabolites production of Anoectochilus formosanus Hayata PLBs were increased by manipulating various culture factors and variable process. The results indicates that MS basal medium with S-3307 1.0mg/L, 6-BA 0.5mg/L and 3% sucrose was found to be equally suitable for biomass accumulation (214.45g/L FW and 18.23g/L DW). Whereas the accumulation of total flavonoids, total phenolics and polysaccharides (5.43mg/g DW, 2.87mg/g DW and 243.23mg/g DW) was maximized at MS medium (NH₄⁺∶NO₃^-ratio is 60∶0mmol/L) supplement with S-3307 1.0mg/L, 6-BA 3.0mg/L and 5% sucrose. The growth pattern was also investigated in suspension culture of PLBs. The maximum biomass production (225.98 g/L FW and 18.53 g/L DW), total flavonoids (5.09mg/g DW) and total phenolics (2.04mg/g DW) were obtained after 7 weeks. However, the polysaccharides production reached its peak (229.36mg/g DW) 5 weeks after suspension culture.

Key words: Anoectochilus formosanus PLBs Secondary metabolites Suspension culture

收稿日期: 2011-12-30 出版日期: 2012-05-25

ZTFLH:

Q813

基金资助:

厦门政府自然科学与技术基金资助项目(3502Z20080012)

通讯作者: 王雅英 E-mail: wangyaying@vip.sina.com

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引用本文:

吕志伟, 吴文平, 毕丽伟, 江星星, 王雅英. 建立金线莲原球茎悬浮体系生产次生代谢产物[J]. 中国生物工程杂志, 2012, 32(05): 43-50.

LV Zhi-wei, WU Wen-ping, BI Li-wei, JIANG Xing-xing, WANG Ya-ying. Establishment of Protocorm-like Bodies of Anoectochilus Formosanus Hayata in Suspension Culture for the Production of Secondary Metabolites. China Biotechnology, 2012, 32(05): 43-50.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/ 或 https://manu60.magtech.com.cn/biotech/CN/Y2012/V32/I05/43

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