L-氨基酸氧化酶的研究进展

中国生物工程杂志

2012, Vol. 32

Issue (03): 125-135

综述

L-氨基酸氧化酶的研究进展

余志良, 周宁, 乔华

浙江工业大学生物与环境工程学院杭州 310014

Advances in L-amino Acid Oxidase

YU Zhi-liang, ZHOU Ning, QIAO Hua

College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou 310014, China

全文: PDF(797 KB) HTML

摘要：

L-氨基酸氧化酶(L-amino acid oxidase, LAAO)能特异性催化L-氨基酸氧化脱氨,生成α-酮酸、氨和H₂O₂。该酶分布较广,其中蛇毒源LAAO是该类酶中研究最为深入的一类,近年来,越来越多的非蛇毒源LAAO被发现和报道,现对蛇毒源和非蛇毒源LAAO的研究进展进行了综述。现有研究表明,不同物种来源的LAAO,其底物选择性、等电点、稳定性等理化性质不尽相同;虽对其结构的研究还较少,但现有的研究表明蛇毒源和非蛇毒源LAAO的结构都含有FAD结合结构域、底物结构域和螺旋结构域;研究已发现不同来源的LAAO体外具有多种不同的生物学功能,而这些生物学功能多数是由于其产物H₂O₂作用的结果;对LAAO异源表达的研究较少且都不甚成功,可能是由于其需要进行翻译后修饰。

关键词： L-氨基酸氧化酶; 生理生化和结构特征; 生物学功能; 异源表达

Abstract:

L-amino acid oxidase is dimeric flavoprotein, and each subunit contains a non-covalently bound FAD molecule as cofactor. It is able to catalyze the stereospecific oxidative deamination of L-amino acids to the corresponding a-imino acids which are then hydrolyzed to corresponding a-keto acids with release of NH₄⁺, along with two electrons transferring from the amino acid to the flavin cofactor which subsequently reduces molecular oxygen to H₂O₂. This enzyme is widely distributed in nature. So far snake venom LAAO is the best characterized member of this enzyme family. Recently, non-snake venom LAAOs have increasingly been found. Current researches show that different LAAOs have different physiological properties, including substrate specificity, pI value, and storage stability. Little is known about its structure, but the structures of both snake venom and non-snake venom LAAOs indicate that it all consists of FAD-binding domain, substrate-binding domain and helical domain. LAAO has various biological functions which are found to be probably related to the produced-H₂O₂. Probably due to post-translational modification of LAAO, only some heterologous expression systems have been reported hitherto.

Key words: LAAO Physiological and structural properties Biological functions Heterologous expression

收稿日期: 2011-09-20 出版日期: 2012-03-25

ZTFLH:

Q55

基金资助:

浙江省自然科学基金(Y5100153)、浙江省公益性应用研究计划(2011C23007)资助项目

通讯作者: 余志良,zlyu@zjut.edu.cn E-mail: zlyu@zjut.edu.cn

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引用本文:

余志良, 周宁, 乔华. L-氨基酸氧化酶的研究进展[J]. 中国生物工程杂志, 2012, 32(03): 125-135.

YU Zhi-liang, ZHOU Ning, QIAO Hua. Advances in L-amino Acid Oxidase. China Biotechnology, 2012, 32(03): 125-135.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/ 或 https://manu60.magtech.com.cn/biotech/CN/Y2012/V32/I03/125

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