木薯<em>ftsZ</em>基因分离及在原核生物中功能初步鉴定

中国生物工程杂志

2013, Vol. 33

Issue (6): 24-29

研究报告

木薯ftsZ基因分离及在原核生物中功能初步鉴定

耿梦婷^1,2, 姚远^1,2, 胡新文¹, 郭建春², 闵义^1,2

1. 海南大学海口 570228;
2. 中国热带农业科学院生物技术研究所海口 571101

Isolation of Cassava ftsZ Plastid Division Family Genes and Preliminary Identification Their Functions in E. coli

GENG Men-tin^1,2, YAO Yuan^1,2, HU Xin-wen¹, GUO Jian-chun², MIN Yi^1,2

1. College of Agriculture, Hainan University, Haikou 570228, China;
2. Institute of Biotechnology and Bioscience, CATAS, Haikou 571101, China

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摘要：

ftsZ基因是控制细胞分裂的关键基因,其蛋白能够在分裂位点形成一个环状结构而影响细胞分裂。为了研究木薯质体分裂与木薯淀粉品质形成的关系,根据木薯基因组数据库上的预测序列,设计引物,从木薯基因组中分离了与质体分裂相关的ftsZ家族3个新基因(ftsZ1, ftsZ2, ftsZ3 )。分别将它们与荧光蛋白基因(GFP)融合,构建了3个原核表达载体pET-ftsZ1-GFP 、pET-ftsZ2-GFP、pET-ftsZ3-GFP,并转化大肠杆菌BL21(DE3)。通过荧光显微镜观察菌体的表型和分裂,初步鉴定了木薯质体分裂相关基因ftsZ家族对细胞分裂的作用。结果显示:尽管木薯与大肠杆菌的亲缘关系较远,ftsZ基因的同源性较低,但是两者表现出相似的功能,木薯ftsZ基因的表达能严重影响大肠杆菌细胞分裂。这一结果为进一步研究木薯ftsZ家族基因的功能奠定了基础。

关键词： 木薯; ftsZ基因家族; 大肠杆菌表达; 细胞分裂

Abstract:

The ftsZ genes are a set of key genes in cell division regulation, and their proteins form a ring structure at the division site to control the process of cell division. In order to study the relationship between cassava starch formation and amyloplast division, three cassava ftsZ family genes were isolated and named as ftsZ1-3. To identify their functions, the ftsZ1-3 genes respectively fused with GFP were expressed in E. coli BL21(DE3). The result showed that the expression of cassava ftsZ genes actively affect the E. coli division although the genetic relationship between cassava and E. coli is far, and the homology of their ftsZ genes is low. This result will be a foundation for further study the function of cassava ftsZ family genes.

Key words: Cassava ftsZ gene family The expression of E. coli Cell division

收稿日期: 2012-10-10 出版日期: 2013-06-25

ZTFLH:

Q781

基金资助:

国家"973计划"(2010CB126600);国家自然科学基金(31160061);木薯现代产业科技体系项目;"211工程"热带作物遗传育种与生态保育创新人才培养基金青年教师项目(QNJS-2011-12)资助项目

通讯作者: 闵义 E-mail: minyi0723@126.com

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引用本文:

耿梦婷, 姚远, 胡新文, 郭建春, 闵义. 木薯ftsZ基因分离及在原核生物中功能初步鉴定[J]. 中国生物工程杂志, 2013, 33(6): 24-29.

GENG Men-tin, YAO Yuan, HU Xin-wen, GUO Jian-chun, MIN Yi. Isolation of Cassava ftsZ Plastid Division Family Genes and Preliminary Identification Their Functions in E. coli. China Biotechnology, 2013, 33(6): 24-29.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/ 或 https://manu60.magtech.com.cn/biotech/CN/Y2013/V33/I6/24

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