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中国生物工程杂志

CHINA BIOTECHNOLOGY
中国生物工程杂志
中国生物工程杂志  2013, Vol. 33 Issue (1): 84-89    
技术与方法     
海洋芽胞杆菌B-9987产新型抗菌环脂肽Marinhysin A的培养基优化
陈杰1, 魏鸿刚1, 罗远婵1, 张道敬1, 李淑兰2, 田黎3, 李元广1
1. 华东理工大学生物反应器工程国家重点实验室 上海 200237;
2. 上海泽元海洋生物技术有限公司 上海 200237;
3. 国家海洋局第一研究所 青岛 266061
Medium Optimization for the Production of New Antifungl Cyclic Lipopeptide Marinhysin A by Bacillus Marinus B-9987
CHEN Jie1, WEI Hong-gang1, LUO Yuan-chan1, ZHANG Dao-jing1, LI Shu-lan2, TIAN Li3, LI Yuan-guang1
1. East China University of Science and Technology, State Key Laboratory of Bioreactor Engineering, Shanghai 200237, China;
2. Shanghai Zeyuan Marine Biotechnology Co., Ltd., Shanghai 200237, China;
3. The First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, China
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摘要： Marinhysin A(MA)是由海洋芽胞杆菌B-9987产生的具有抗真菌活性的新结构环脂肽类化合物,该化合物不仅抑菌谱广而且盆栽防效良好。采用响应面分析法(Response Surface Methnology)对海洋芽胞杆菌B-9987产脂肽MA的培养基进行了优化。Plackett-Burman (PB)实验表明,蔗糖和酵母粉是显著影响MA产量的因素。中心组合实验(Central Composite Design)分析表明,当培养基中蔗糖和酵母粉的含量分别为42.0g/L和42.3g/L时,MA的浓度最大可达68.19mg/L,与实验值75.74mg/L相近。优化后,MA产量(250ml摇瓶发酵)较优化前的54.12mg/L提高了28.5%。用优化后培养基进行5L罐发酵,MA浓度可达182mg/L,较优化前的130mg/L 提高了28.4%。
关键词： Marinhysin A;  海洋芽胞杆菌B-9987;  培养基优化;  响应面分析法    
Abstract: The new antifungal cyclic lipopeptide Marinhysin (MA) produced by Bacillus marinus B-9987 shows the excellent activity against plant pathogens both in vitro and in vivo. In the present study, the response surface methodology (RSM) was employed to optimize the medium composition for producing MA. The results of the Plackett-Burman design showed that sucrose and yeast powder had significant effects on MA production. The central composite design was applied to further optimize the concentration of sucrose and yeast powder in the medium. The results of analysis suggested that the optimum values of the tested variables were sucrose of 42.0 g/L and yeast powder of 42.3 g/L. MA concentration reached 75.74 mg/L, which was in agreement with the predicted value of 68.19mg/L. In comparison to the production of original level (54.12mg/L) in a 250ml flask, 1.28-fold increment had been obtained. By scaling u Pthe fermentation from flask to a 5L fermentor with the optimal medium, MA concentration was increased to 182mg/L, which was 1.28 times of the value (130mg/L) with the original medium.
Key words: Marinhysin A    Bacillus marinus B-9987    Medium optimization    Response surface methodology
收稿日期: 2012-11-01 出版日期: 2013-01-25
ZTFLH:  Q815  
基金资助: 国家"863"计划(2011AA09070402,2011AA10A202-2,2011AA10A205); 国家科技支撑计划(2011BAE06B04-16)资助项目
通讯作者: 李元广     E-mail: ygli@ecust.edu.cn
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陈杰
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田黎
李元广

引用本文:

陈杰, 魏鸿刚, 罗远婵, 张道敬, 李淑兰, 田黎, 李元广. 海洋芽胞杆菌B-9987产新型抗菌环脂肽Marinhysin A的培养基优化[J]. 中国生物工程杂志, 2013, 33(1): 84-89.

CHEN Jie, WEI Hong-gang, LUO Yuan-chan, ZHANG Dao-jing, LI Shu-lan, TIAN Li, LI Yuan-guang. Medium Optimization for the Production of New Antifungl Cyclic Lipopeptide Marinhysin A by Bacillus Marinus B-9987. China Biotechnology, 2013, 33(1): 84-89.

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https://manu60.magtech.com.cn/biotech/CN/    或    https://manu60.magtech.com.cn/biotech/CN/Y2013/V33/I1/84

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