技术与方法 |
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抗心型脂肪酸结合蛋白单克隆抗体制备、鉴定和在侧向免疫层析方法中的应用 |
康可人1,2, 吴培钿2, 李悦琳2, 黄绮玲2, 李高辉2, 唐时幸2, 王继华2 |
1. 华南理工大学生物科学与工程学院 广州 510660; 2. 自检型快速诊断国家地方联合工程实验室 广州万孚生物技术股份有限公司 广州 510663 |
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Development and Characterization of Monoclonal Antibodies Against Human Heart Type Fatty Acid Binding Protein and Its Application in Lateral Flow Immunoassay |
KANG Ke-ren1,2, WU Pei-dian2, HUANG Qi-lin2, LI Yue-lin2, LI Gao-hui2, TANG Shi-xing2, WANG Ji-hua2 |
1. School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510006, China; 2. National Engineering Laboratory of Rapid Diagnostic Test. Guangzhou Wondfo Biotech Co. Ltd., Guangzhou 510663, China |
引用本文:
康可人, 吴培钿, 李悦琳, 黄绮玲, 李高辉, 唐时幸, 王继华. 抗心型脂肪酸结合蛋白单克隆抗体制备、鉴定和在侧向免疫层析方法中的应用[J]. 中国生物工程杂志, 2013, 33(1): 72-78.
KANG Ke-ren, WU Pei-dian, HUANG Qi-lin, LI Yue-lin, LI Gao-hui, TANG Shi-xing, WANG Ji-hua. Development and Characterization of Monoclonal Antibodies Against Human Heart Type Fatty Acid Binding Protein and Its Application in Lateral Flow Immunoassay. China Biotechnology, 2013, 33(1): 72-78.
链接本文:
https://manu60.magtech.com.cn/biotech/CN/
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https://manu60.magtech.com.cn/biotech/CN/Y2013/V33/I1/72
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[1] Ockner R K, Manning J A, Poppenhausen R B, et al. A bindingprotein for fatty acids in cytosol of intestinal mucosa, liver, myocardium,and other tissues. Science, 1972, 177(4043):56-58. [2] Su A I, Wiltshire T, Batalov S, et al. A geneatlas of the mouse and human protein-encodingtranscriptomes. ProcNatlAcadSci USA 2004, 101(16):6062-6067 [3] Schaap F G, Binas B, Danneberg H, et a1. Impaired long-chain fatty acid utilization by cardiac myocytes isolated from mice lacking the heart-type fatty acid binding protein gene. Circ Res, 1999, 85(4):329-337. [4] Tanaka T, Hirota Y, Sohmiya K, et al. Serum and urinary human heart fatty acid-binding protein in acute myocardial infarction. Clin Biochem, 1991, 24(2):195-201. [5] Pelsers M M, Hermens W T, Glatz J F. Fatty acid-binding proteins asplasma markers of tissue injury. ClinChimActa, 2005, 352(1-2):15-35. [6] Chan C P, Sanderson J E, Glatz J F, et al. A superior early myocardial infarction marker. Human heart-type fatty acid-binding protein. Z Kardiol, 2004, 93(5):388-397. [7] Bertinchant J P, Polge A. Diagnostic and prognostic value of heart-type fatty acid-binding protein (H-FABP), an early biochemical marker of myocardial injury. Arch Mal Coeur Vaiss, 2005, 98(12):1225-1231. [8] Alhadi H A, Fox K A. Do we need additional markers of myocytenecrosis: the potential value of heart fatty-acid-binding protein. QJM, 2004, 97(4):187-198. [9] Raghava G P, Agrewala J N. Method for determining the affinity of monoclonal antibody using non-competitive ELISA: a computer program. J Immunoassay, 1994, 15(2):115-128. [10] Ghani F, Wu A H, Graff L, et al. Role of heart-type fatty acid-binding protein in early detection of acute myocardial infarction. Clin Chem, 2000, 46(5):718-719. [11] Wu A H. Analytical and clinical evaluation of new diagnostic tests for myocardial damage. Clin Chim Acta, 1998, 272(1):11-21. [12] Trifonov I R, Katrukha A G, Deev A D,et al. Heart fatty acid binding protein in patients with ST-elevation acute coronary syndrome hospitalized within 6 hours after onset of pain. Kardiologiia, 2002, 42(6):18-23. [13] Nakata T, Hashimoto A, Hase M,,et al. Human heart-type fatty acid-binding protein as an early diagnostic and prognostic marker in acute coronary syndrome. Cardiology, 2003, 99(2):96-104. [14] Suzuki M, Hori S, Noma S, et al. Prognostic value of a qualitative test for heart-type fatty acid-binding protein in patients with acute coronary syndrome. Int Heart J, 2005, 46(4):601-606. [15] Roos W, Eymann E, Symannek M, et al. Monoclonal antibodies to human heart fatty acid-binding protein. J Immunol Methods, 1995, 183(1):149-153. |
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