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中国生物工程杂志

CHINA BIOTECHNOLOGY
中国生物工程杂志  2010, Vol. 30 Issue (02): 120-126    DOI: Q78
综述     
转基因植物检测技术的研究进展
郭斌,祁洋,尉亚辉**
西北大学生命科学学院 西安 710069
The Advances of Transgenic Plants Detection Techniques
College of Life Science, Northwest University, Xi’an 710069,China
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摘要:

现代植物基因工程使转基因植物及其产品越来越多地进入人们的生活,转基因植物安全性在世界范围内引起了广泛关注,对转基因植物检测技术的需求也越来越紧迫。就转基因植物检测技术的研究进展进行综述,重点介绍以基因和蛋白为目标的检测技术,包括PCR、ELISA和基因芯片技术的最新进展,并对不同方法的优缺点进行对比。此外,提出对特定代谢产物的检测是转基因植物检测的重要组成部分,是以后检测技术的发展趋势之一。最后,以差异蛋白为检测目标,结合研究工作提出基于双向电泳技术的转基因植物检测方法及其产品溯源方案。

关键词: 转基因植物检测技术基因芯片PCR双向电泳    
Abstract:

With the development of genetic engineering, more and more transgenic plants and their products released to people's lives and greatly changed our lives. The controversy over potential ecological risks and the impact of transgenic plants on human health are becoming increasingly acute. Therefore, the techniques for detecting transgenic plants must be established and perfected.The advances in detection techniques of transgenic plants were summarized. The progresses of PCR, ELISA as well as gene chip in transgenic plants detection were emphasized,and their characteristics were compared. Furthermore, detection on specific metabolites in transgenic plants was also introduced. Based on detection of the differential protein, one project of two-dimensional electrophoresis technology for traceability of genetically modified organisms and products was put forward finally.

Key words: Transgenic plants    Detection techniques    Gene chipPCR    Two-dimensional electrophoresis
收稿日期: 2009-10-21 出版日期: 2010-02-26
基金资助:

陕西省科技专项(20081206)资助项目

通讯作者: 尉亚辉     E-mail: weiyahui@nwu.edu.cn
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引用本文:

郭斌 祁洋 尉亚辉. 转基因植物检测技术的研究进展[J]. 中国生物工程杂志, 2010, 30(02): 120-126.

GUO Bin, QI Xiang, WEI E-Hui. The Advances of Transgenic Plants Detection Techniques. China Biotechnology, 2010, 30(02): 120-126.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/Q78        https://manu60.magtech.com.cn/biotech/CN/Y2010/V30/I02/120

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