基于MegaBACE 1000的荧光(marker)开发

中国生物工程杂志

2009, Vol. 29

Issue (12): 90-93

技术与方法

基于MegaBACE 1000的荧光(marker)开发

刘贤德^1,2, 张国范^1**

1.中国科学院海洋研究所青岛 266071
2.福建省高校水产科学技术与食品安全重点实验室/集美大学水产学院厦门 361021

The Fluorescent Marker Development Based on MegaBACE 1000 DNA Sequencing System

LIU Xian-de^1,2,ZHANG Guo-fan¹

1.Chinese Academy of Sciences, Institute of Oceanology, Qingdao 266071, China
2.Key Laboratory of Science and Technology for Aquaculture and Food Safety of Fujian Province University,Fisheries College, Jimei University, Xiamen 361021, China

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摘要：

MegaBACETM 1000 DNA测序仪是由美国Pharmacia公司生产的一种高通量的DNA测序仪，利用这套测序仪，可以进行DNA测序、遗传分析等相关研究。但该公司生产的用于遗传分析的DNA标记物（marker）（ET550-R Size Standards）价格不菲，出于节省成本的考虑，自行开发了荧光标记物，经过验证，这个标记完全可以在MegaBACE 1000 DNA测序仪上使用。利用这套标记物和自己开发的标记物识别软件，构建了一套基于MegaBACE 1000 DNA测序仪的改良的、高通量的AFLP操作流程。

关键词： MegaBACE; marker;; AFLP

Abstract:

The MegaBACETM 1000 DNA Sequencing System was made by Amersham Pharmacia, which is a high throughput fluorescence-based DNA sequencer that can be used for DNA sequencing and genetic analysis etc. But the markers（ET550-R Size Standards）produced by this corporation which are used for genetic analysis in this instrument are very expensive. In order to cut the cost,a set of markers were developed and after authentication,that can be used in the MegaBACE 1000 DNA sequencer. Using these markers and our own recognition software,an improved, high throughput AFLP work protocol based on MegaBACETM 1000 were constructed.

Key words: MegaBACE Marker AFLP

收稿日期: 2009-05-07 出版日期: 2009-12-21

ZTFLH:

Q819

基金资助:

国家“863”计划（2006AA10A407）、福建省青年人才项目（2007F3074）资助项目

通讯作者: 张国范 E-mail: gfzhang@ms.qdio.ac.cn

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引用本文:

刘贤德张国范. 基于MegaBACE 1000的荧光(marker)开发[J]. 中国生物工程杂志, 2009, 29(12): 90-93.

LIU Xian-De, ZHANG Guo-Fan. The Fluorescent Marker Development Based on MegaBACE 1000 DNA Sequencing System. China Biotechnology, 2009, 29(12): 90-93.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/ 或 https://manu60.magtech.com.cn/biotech/CN/Y2009/V29/I12/90

［1］	Liu X D, Liu X, Guo X, et al. A preliminary genetic linkage map of the pacific abalone Haliotis discus hannai Ino. Marine Biotechnology, 2006, 8: 386~397
［2］	GeneScan Reference Guide, Chemistry Reference for the ABI Prism 310 genetic Analyzer. PE applied system. Chapter 5, 1~17
［3］	MegaBACE 1000 Instrument Administrator’s Guide Version 2.0, Amersham Biosciences, Appendix A Fluorescence imaging, pA1~11
［4］	Igor L Medintz, Lorenzo Berti, Charles A Emrich, et al. Genotyping energytransfercassettelabeled ShortTandemRepeat Amplicons with Capillary Array Electrophoresis Microchannel Plates, Clinical Chemistry,2001, 47(9):1614~1621

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