实时荧光PCR检测食品中致敏原芥末成分

中国生物工程杂志

2011, Vol. 31

Issue (01): 61-64

技术与方法

实时荧光PCR检测食品中致敏原芥末成分

邵娟¹, 曹际娟², 刘洋², 王长文², 赵彤彤², 李晶泉³

1. 大连工业大学生物工程学院大连 116034;
2. 辽宁出入境检验检疫局大连 116001;
3. 宝生物工程(大连有限公司大连 116600

Detection of Allergen Mustard Components in Food by Real-time Fluorescent PCR

SHAO Juan¹, CAO Ji-juan², LIU Yang², WANG Chang-wen², ZHAO Tong-tong², LI Jing-quan³

1. School of Biological Engineering, Dalian Polytechnic University, Dalian 116034, China;
2. Liaoning Entry-Exit Inspection & Quarantine Bureau, Dalian 16001, China;
3. TaKaRa Biotechnology(Dalian CO., LTD., Dalian 116600, China

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摘要：

对植物原料产品和植物源性食品中芥末成分的快速鉴定是避免过敏性疾病发生的重要措施。依据芥末Sin A1管家基因的核酸序列设计特异性引物和探针,对3种芥末样品和21种非芥末植物样品进行实时荧光PCR检测,结果显示,过敏原芥末管家基因的样品FAM通道有荧光信号检出,非芥末样品FAM通道均无荧光信号检出。灵敏度实验表明,植物原料产品中对芥末的检测低限可达到1 mg/kg。此外对市售的芥菜籽等样品和深加工的芥末致敏原参考物质(葡萄糖)进行实际样品的检测,均能很好检出致敏原芥末成分。

关键词： 实时荧光PCR; 芥末; 过敏原; 管家基因

Abstract:

Rapid identification of mustard materials in plant raw material products and plant-based foods is essential for effective control of a potential source of Allergen pathogens. A convenient Real-Time fluorescence polymerase chain reaction (PCR)-based assay which allowed detection and identification of a mustard -specific Housekeeping gene sin AI DNA sequence in foodstuffs and food was developed. The experiment results show that: the primers and probes could specific identify three kinds of mustard with 21 kinds of samples. Sensitivities results show that: 1 mg/kg for mustard of foods could be detected. Moreover, the four commercial samples (including mustard seeds) and deep processing of mustard allergen reference material (glucose) were detected and the detection results show that mustard allergen components were detected well.

Key words: Real-time PCR Mustard Allergen Housekeeping gene

收稿日期: 2010-09-10 出版日期: 2011-01-25

ZTFLH:

Q789

基金资助:

国家公益性行业科研专项课题资助项目(2007GYJ036,10-46)

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引用本文:

邵娟, 曹际娟, 刘洋, 王长文, 赵彤彤, 李晶泉. 实时荧光PCR检测食品中致敏原芥末成分[J]. 中国生物工程杂志, 2011, 31(01): 61-64.

SHAO Juan, CAO Ji-juan, LIU Yang, WANG Chang-wen, ZHAO Tong-tong, LI Jing-quan. Detection of Allergen Mustard Components in Food by Real-time Fluorescent PCR. China Biotechnology, 2011, 31(01): 61-64.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/ 或 https://manu60.magtech.com.cn/biotech/CN/Y2011/V31/I01/61

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