| 研究报告 |
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| 多拉菌素产生菌aveD基因缺失突变株的构建 |
| 王洁颖1,2,甘邱锋2,3,张晓琳2**,汪洋2,宋渊4,路福平1 |
1.天津科技大学工业微生物教育部重点实验室 天津 300457
2.国家粮食局科学研究院 北京 100037
3.福建农林大学生物农药与化学生物学教育部重点实验室 福州 350002
4.中国农业大学生物学院微生物系 北京 100094 |
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| Deletion Analysis of aveD Gene from a Streptomyces avermitilis Mutant Producing Doramectin |
1. Key Laboratory of Industrial Microbiology, Ministry of Education, Tianjin University of Science and Technology, Tianjin 300457, China
2.Academy of State Administration of Grain, Beijing 100037, China
3.Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education,Fujian Agriculture and Forestry University, Fuzhou 350002, China
4.Department of Microbiology, College of Biological Sciences, China Agricultural University, Beijing 100094, China |
| ［1］ Burg R W, Miller B M, Baker E E, et al. Avermectins, new family of potent anthelmintic agents: producing organism and fermentation. Antimicrob Agents Chemother, 1979, 15(3): 361367.
［2］ Ikeda H, Omura S. Control of avermectin biosynthesis in Streptomyces avermitilis for the selective production of a useful component. J Antibiot, 1995, 48(7): 549562.
［3］ Ikeda H, Nonomiya T, Usami M, et al. Organization of the biosynthetic gene cluster for the polyketide anthelmintic macrolide avermectin in Streptomyces avermitilis. Proc Natl Acad Sci USA, 1999, 96(17): 95099514.
［4］ Ikeda H, Ishikawa J, Hanamoto A, et al. Complete genome sequence and comparative analysis of the industrial microorganism Streptomyces avermitilis. Nat Biotechnol, 2003, 21(5): 526531.
［5］ Denoya C D, Fedechko R W, Hafner E W, et al. A second branchedchain alphaketo acid dehydrogenase gene cluster(bkdFGH) from Streptomyces avermitilis: its relationship to avermectin biosynthesis and the construction of a bkdF mutant suitable for the production of novel antiparasitic avermectins. J Bacteriol, 1995, 177(12): 35043511.
［6］ 姜薇, 汪洋, 张晓琳, 等. 阿维菌素衍生物CHCB1的突变生物合成. 中国生物工程杂志, 2009, 29(8): 6874. Jiang W, Wang Y, Zhang X L, et al.China biotechnology, 2009, 29(8): 6874.
［7］ Cropp T A, Wilson D J, Reynolds K A. Identification of a cyclohexylcarbonyl CoA biosynthetic gene cluster and application in the production of doramectin. Nat Biotechnol, 2000, 18(9): 980983.
［8］ 房春林, 杨光有, 古小彬, 等. 多拉菌素的研究进展. 中国畜牧兽医, 2006, 33(5): 5557. Fang C L, Yang Y G, Gu X B, et al.China Anim Hus Vet Med, 2006, 33(5): 5557.
［9］ Ikeda H, Wang L R, Ohta T, et al. Cloning of the gene encoding avermectin B 5Omethyltransferase in avermectinproducing Streptomyces avermitilis. Gene, 1998, 206(2): 175180.
［10］ Bierman M, Logan R, O′Brien K, et al. Plasmid cloning vectors for the conjugal transfer of DNA from Escherichia coli to Streptomyces spp. Gene, 1992, 116(1): 4349.
［11］ Hanahan D. Studies on transformation of Escherichia coli with plasmids. J Mol Biol,1983, 166(4): 557580.
［12］ Sambrook J, Russell D. Molecular Cloning: A Laboratory Manual. 3rd ed. New York: Cold Spring Harbor Laboratory Press, 2001.
［13］ MacNeil D J, Klapko L M. Transformation of Streptomyces avermitilis by plasmid DNA.J Ind Microbiol, 1987, 2(4): 209218.
［14］ Ikeda H, Kotaki H, Tanaka H, et al. Involvement of glucose catabolism in avermectin production by Streptomyces avermitilis. Antimicrob Agents Chemother, 1988, 32(2): 282284.
［15］ Hopwood D A, Bibb M J, Chater K F, et al. Genetic manipulation of Streptomyces: A Laboratory Manual. Norwich: John Innes Foundation, 1985.
［16］ 姜薇. 多拉菌素的突变生物合成及发酵条件优化. 哈尔滨: 东北农业大学, 生命科学学院，2009. Jiang W. The mutational biosynthesis of doramectin and studies on the fermentation condition. Harbin: Northeast Agricultural University, College of life Sciences,2009.
［17］ 陈芝, 文莹, 宋渊, 等. 阿维链霉菌中aveD基因缺失对阿维菌素合成的影响. 微生物学报, 2002, 42(5): 534538. Chen Z, Wen Y, Song Y, et al.Acta Microbiol Sin, 2002, 42(5): 534538.
［18］ StutzmanEngwall K, Conlon S, Fedechko R, et al. Engineering the aveC gene to enhance the ratio of doramectin to its CHCB2 analogue produced in Streptomyces avermitilis. Biotechnol Bioeng, 2003, 82(3): 359369. |
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