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中国生物工程杂志

CHINA BIOTECHNOLOGY
中国生物工程杂志  2011, Vol. 31 Issue (04): 25-30    
研究报告     
经NGR肽段遗传修饰的携带三种报告基因的腺病毒载体的构建及其实验研究
冯飞雪, 赵俊丽, 王东阳, 刘世海, 王丽娴, 夏海滨
陕西师范大学生命科学学院基因治疗研究室 西安 710062
Construction of NGR Peptide Genetically Modified Adenovirus Vector Expressing Three Reporter Genes and Its Experimental Study
FENG Fei-xue, ZHAO Jun-li, WANG Dong-yang, LIU Shi-hai, WANG Li-xian, XIA Hai-bin
Laboratory of Gene Therapy, College of Life Sciences, Shaanxi Normal University, Xi’an 710062,China
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摘要:

NGR(Asn-Gly-Arg)是通过噬菌体展示技术筛选出来的能够和肿瘤新生血管特异结合的三肽模体,可以将多种药物分子和病毒载体靶向运输到肿瘤或者进行血管再生的组织中。为此构建了腺病毒衣壳蛋白knob的HI环(HI-loop)经NGR肽段修饰的并同时表达三种报告基因的腺病毒载体Ad5/E1-mCherry/E3-luciferase-2A-eGFP/knob-NGR。体内、外实验研究表明,该病毒载体可成功表达三种报告基因;经NGR肽段修饰的腺病毒载体对人乳腺癌细胞系MDA-MB-231的感染效率高于未经修饰的对照腺病毒Ad5CMVeGFP。该载体的成功构建为进一步研究经NGR肽段修饰的腺病毒在肿瘤动物模型体内的靶向性及经NGR肽段修饰的并携带治疗基因的实验治疗研究奠定了基础。

关键词: 腺病毒载体NGR肽段衣壳蛋白遗传修饰肿瘤基因治疗    
Abstract:

NGR (Asn-Gly-Arg) motif exploited by phage display can selectively recognize neovasculature, and can be used for ligand-directed targeted delivery of various drugs and viral vectors to tumors or to other tissues with an angiogenesis process. Therefore,constructed the adenoviral vector Ad5/E1-mCherry/E3-luciferase-2A-eGFP/fiber-NGR which was modified with NGR peptide in the HI loop of the knob and could express three reporter genes. The study of the adenovirus in vitro and in vivo indicated that the three reporter genes could be expressed successfully and the infection efficiency indicated human breast cancer cell line MDA-MB-231 mediated by constructed adenovirus was higher than that mediated by unmodified control adenovirus Ad5CMVeGFP. The construction of the adenoviral vector laid a foundation for the further research on the target of adenoviral vector modified with NGR peptide and the therapy research of adenoviral vector carrying therapeutic genes in tumor animal model.

Key words: Adenoviral vector    NGR peptide    Caspid genetically modification    Tumor gene therapy
收稿日期: 2010-11-08 出版日期: 2011-04-26
ZTFLH:  Q789  
基金资助:

国家自然科学基金(30872993)资助项目

通讯作者: 夏海滨     E-mail: hbxia2001@163.com
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引用本文:

冯飞雪, 赵俊丽, 王东阳, 刘世海, 王丽娴, 夏海滨. 经NGR肽段遗传修饰的携带三种报告基因的腺病毒载体的构建及其实验研究[J]. 中国生物工程杂志, 2011, 31(04): 25-30.

FENG Fei-xue, ZHAO Jun-li, WANG Dong-yang, LIU Shi-hai, WANG Li-xian, XIA Hai-bin. Construction of NGR Peptide Genetically Modified Adenovirus Vector Expressing Three Reporter Genes and Its Experimental Study. China Biotechnology, 2011, 31(04): 25-30.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/        https://manu60.magtech.com.cn/biotech/CN/Y2011/V31/I04/25


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