中国生物工程杂志

Objective:Set a new chromatography method to remove impurity proteins from meningococcal polysaccharide type A sample instead of  the traditional phenol abstraction method. Method:This new method includes two purification steps by using Capto adhere and Sephardex G-25. Result:The contaminative protein concentration is 0.2% in final meningococcal polysaccharise type A sample, which is below the WHO regulation standard (1%),the content of nucleic acid and endotoxin are also blow the current regulation standard,the recovery is over 65%. Conclusion:Compare with the tradition phenol abstraction method, the chromatography method can remove impurity proteins effectively with high recovery and reducing environment pollution and easy to scale up.