技术与方法 |
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急性早幼粒白血病HL60细胞电转染条件的优化 |
王伟佳1,张秀明1**,王前2,温冬梅1,邱宗荫3 |
1.中山市人民医院,南方医科大学 广州 528402
2.南方医科大学附属南方医院 广州 510515
3.重庆医科大学 重庆 400016 |
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A Suitable Method for Electroporation of Human Acute-premyelocyte HL60 Cell Line |
WANG Wei-jia1,ZHANG Xiu-ming1,WANG Qian2,WEN Dong-mei1,QIU Zong-yin3 |
1.Zhongshan People’s Hospital, Nanfang Medical University,Guanzhou 528402, China
2.The Affiliated Hospital of Nanfang University, Guangzhou510515, China
3.Chongqing Medical University, Chongqing400016, China |
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[2] Frank S, Peter B, Martin M, et al. Novel nonviral method for transfection of primary leukemia cells and cell lines. Genetic Vaccines and Therapy, 2004,2:1.
[3] 薛芳,成志勇,杨琳,等.白血病耐药细胞系U937/ADR的建立及其生物学性状.中国生物工程杂志,2009,29(7):1721. Xue F, Cheng Z Y, Yang L, et al.China Biotechnology, 2009,29(7):1721.
[4] Jeong A K, Keunchang C, Mi S S, et al. A novel electroporation method using a capillary and wiretype electrode. Biosensors and Bioelectronics, 2008, 23:13531360.
[5] Melkonyan H, Sorg C, Klempt M. Electroporation efficiency in mammalian cells is increased by dimethyl sulfoxide (DMSO). Nucleic Acids Research, 1996,24:43564357.
[6] Chuang S E, Chen A L, Chao C C, et al. Growth of E.coli at low temperature dramatically increases the transformation frequency by electroporation. Nucleic Acids Res, 1995,23:1641.
[7] Hong N Y, Young R L, Eun M N, et al. Tumor necrosis factorα enhances DMSOinduced differentiation of HL60 cells through the activation of ERK/MAPK pathway. International Journal of Hematology, 2008,87:189194. |
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