研究报告 |
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大肠杆菌耐热性肠毒素STI三重串联突变体与黏附素K99融合基因的表达研究 |
郭乐,李敏,王玉炯** |
宁夏大学生命科学学院 西部特色生物资源保护与利用教育部重点实验室 银川 750021 |
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Expression of fusion gene of tree Heat-stable Toxin I’ mutants and K99 adhesin of Enterotoxigenic Escherichia coli |
GUO Le,LI Min,WANG Yu-jiong |
Key Laboratory of Ministry of Education for Protection and Utilization of Special Biological Resource in Western China,School of Life Science,Ningxia University,Yinchuan 750021,China |
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[3] Moseley S L, Hardy J M, Huq M I, et al. Isolation and nucleotide sequence determination of a gene encoding a heatstable enterotoxin of Escherichia coli. Infect Immun, 1983, 39:11671174.
[4] Roosendaal B, Gaastra W, De Graaf F K. The nucleotide sequence of the gene encoding the K99 subunit of enterotoxigenic Escherichia coli.FEMS Microbiol Letters, 1984, 22:253258.
[5]Sambrook J, Fritsch E F, Maniatis T. Molecular Cloning: A Laboratory Manual.2nd ed. New York: Cold Spring Harbor Laboratory Press,1989.2138.
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[7] Clements J D. Construction of a nontoxic fusion peptide for immunization against Escherichia coli strains that produce heatlabile and heatstable enterotoxins. Infect Immun, 1990, 58(1):159. |
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