多聚嘧啶序列结合蛋白（PTB）与HBV转录后调节元件（HPRE）结合抑制HBV表面抗原的基因表达

中国生物工程杂志

2009, Vol. 29

Issue (02): 24-28

研究报告

多聚嘧啶序列结合蛋白（PTB）与HBV转录后调节元件（HPRE）结合抑制HBV表面抗原的基因表达

程丽英¹,张小花¹,李毅¹,蔡雪飞¹,胡源¹,黄爱龙¹,汤华²

1. 重庆医科大学感染性疾病分子生物学教育部重点实验室
2. 重庆医科大学

Polypyrimidine Tract Binding Protein Negatively Regulates the Expression of HBV Surface Antigen by Interacting with HBV Postranscriptional Regulatory Element

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摘要： 采用RT-PCR验证PTB与HPRE在体外的特异性结合。用HepG2.2.15细胞系、HBs-HPRE瞬时转染Hela细胞探讨PTB对HBV基因表达的影响。结果显示PTB能与HPRE特异性地结合。功能性研究证明PTB可以抑制HepG2.2.15细胞的HBsAg表达量，并呈浓度依赖性。由HPRE引起的HBsAg表达的增加也能被PTB所抑制。实验数据证明PTB通过与HPRE相互作用抑制HBsAg的基因表达。

关键词： 乙型肝炎病毒;HBV转录后调节元件（HPRE）;多聚嘧啶序列结合蛋白（PTB）;HBV;表面抗原;基因表达

Abstract: In order to demonstrate PTB bind to HPRE, reverse transcription, PCR-mediated detection, were used. HepG2.2.15 cell line and HBs-HPRE transient expression cells were adopted to identify PTB function in HBV life cycle.The results showed that PTB could directly bind to HPRE-RNA. Functional analysis indicated that PTB could inhibit the expression of HBs antigen and this inhibition was in a dosedependent manner in HepG2.2.15 cells. Higher expression of HBs in cells transfected pcDNA3HBsHPRE comparing with pcDNA3HBs, and this high expression could also be inhibited by PTB.The data demonstrated that PTB inhibits HBs expression by interacting with HPRE.

收稿日期: 2008-10-14 出版日期: 2009-03-31

ZTFLH:

R373

基金资助:

国家自然科学基金

通讯作者: 汤华

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引用本文:

程丽英,张小花,李毅,蔡雪飞,胡源,黄爱龙,汤华. 多聚嘧啶序列结合蛋白（PTB）与HBV转录后调节元件（HPRE）结合抑制HBV表面抗原的基因表达[J]. 中国生物工程杂志, 2009, 29(02): 24-28.

CHENG Li-Yang- Zhang-Xiao-Hua- Li-Yi- Ca-Xue-Fei- Hu-Yuan- Huang-Ai-Long- Shang-Hua. Polypyrimidine Tract Binding Protein Negatively Regulates the Expression of HBV Surface Antigen by Interacting with HBV Postranscriptional Regulatory Element. China Biotechnology, 2009, 29(02): 24-28.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/ 或 https://manu60.magtech.com.cn/biotech/CN/Y2009/V29/I02/24

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