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中国生物工程杂志

CHINA BIOTECHNOLOGY
中国生物工程杂志  2013, Vol. 33 Issue (1): 47-52    
研究报告     
一株苯系物降解真菌筛选及降解特性
张占生1, 李彦旭1, 杜青平1, 夏丽娟2
1. 广东工业大学环境科学与工程学院 广州 510006;
2. 广东工业大学轻工化工学院 广州 510006
Isolation of a Fungal Strain of Degrading BTEX and Its Degradation Characteristics
ZHANG Zhan-sheng1, LI Yan-xu1, DU Qing-ping1, XIA Li-juan2
1. School of Environmental Science and Engineering, Guangdong University of Technology,Guangzhou 510006, China;
2. School of Chemical Engineering and Light Industry, Guangdong University of Technology,Guangzhou 510006, China
 全文: PDF(821 KB)   HTML
摘要: 采用逐量分批驯化的方法以污水处理厂污泥作为菌源,苯、甲苯、二甲苯为唯一碳源,驯化、分离、筛选能够有效降解苯系物的真菌,命名为B1。采用单因素以及正交实验方法并对真菌降解环境影响因素及降解效率进行了测定和研究。结果表明:真菌B1对苯系物降解的最佳条件为C:N=5:1,pH5,温度30℃,菌种接种量为5.5ml(50ml培养基)。采用GC对初始液相浓度0~90mg/L范围内的苯系物降解效果进行测定,未发现苯系物对真菌降解活性产生抑制作用。真菌对苯系物的降解效率为:甲苯>苯>二甲苯,最高降解效率分别达到87.39%,85.21%,81.47%。混合物降解效果略高于单一底物的降解效果。
关键词: 甲苯二甲苯真菌降解效率    
Abstract: By gradually increasing the concentration of BTEX in the culture medium with toluene, benzene and xylene as the sole carbon source, a BTEX-degrading fungi strain which was designated as B1 was isolated from the sludge of sewage treatment plant. Using single factor and orthogonal experiment analyzed and studied the fungal degradation environment effect factors and degradation efficiency. The results showed that the optimum condition of fungal degradation was C:N = 5:1, pH5, temperature 30℃, strains inoculation amount 5.5ml (50ml culture medium). GC analysis was used to determine the fungal degradation effect to BTEX with the initial liquid phase concentration of 0~90mg/L, the results showed that the degradation activities of fungal strain was not inhibited, and the fungal degradation efficiency was toluene > benzene > xylene, the highest degradation efficiency reached 87.39%, 85.21%, 81.47%,respectively. The degradation efficiency of mixed substrates was slightly higher than single substrate.
Key words: Toluene    Tenzene    Xylene    Fungal    Degradation efficiency
收稿日期: 2012-09-03 出版日期: 2013-01-25
ZTFLH:  Q93  
基金资助: 国家自然科学基金资助项目(40971251)
通讯作者: 李彦旭     E-mail: 491559713@qq.com
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引用本文:

张占生, 李彦旭, 杜青平, 夏丽娟. 一株苯系物降解真菌筛选及降解特性[J]. 中国生物工程杂志, 2013, 33(1): 47-52.

ZHANG Zhan-sheng, LI Yan-xu, DU Qing-ping, XIA Li-juan. Isolation of a Fungal Strain of Degrading BTEX and Its Degradation Characteristics. China Biotechnology, 2013, 33(1): 47-52.

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https://manu60.magtech.com.cn/biotech/CN/        https://manu60.magtech.com.cn/biotech/CN/Y2013/V33/I1/47

[1] Cui B W,Wang L Q,Sun J J. Determinatoin of toluene and benzene in water by the wide-bore capillary column GC and the head-space sampler. Journal of Analytical Science, 2003, 19(5): 488-489.
[2] 卓黎阳,舒小平,郑海涛,等. 顶空进样气相色谱法分析溶剂型木器涂料中的苯系物. 福建分析测试, 2008,17(4):40-43. Zhuo L Y, Shu X P, Zheng H T,et al.Determination of benzne, toluene and Xylene in solvent coatings for woodenware by headspace-GC.Fujian Analysis & Testing.2008,17(4):40-43.
[3] Zhou J Z,Wang X L, Cao H R,et al. Benzene series in oil paint thinners determined by gas chromatography. Environmental Science & Technology, 2007, 30(1): 19-21.
[4] Zhaohui Xu A M W C. Detection of Benzene,Toluene,Ethyl benzene,and Xylenes (BTEX) Using Toluene Dioxygenase-Peroxidase Coupling Reactions, 2003: 19, 1812-1815.
[5] Inoue A;Horikoshi K A. Pseudomonas thrives in high concentrations of toluene.Nat,1989,338:264-266.
[6] 周婷,袁世斌,邹晓莉. 一株踝节霉菌的培养特性及其在污泥生物淋滤中的应用. 应用与环境生物学报, 2012(1): 128-133. Zhou T, Yuan S B, Zou X L, Cultural characteristics of a strain of Talaromyces sp.and its application in bioleaching of sewage sludge.Chinese Journal of Applied & Environmental Biology.2012,18(1):128-133.
[7] 李玮娜. 真菌生物处理"三苯"废气研究. 西安科技大学, 2007. Li W.Studies on the Treatment of Off-gases Containing BTX with Fungi.Xi'an University of Science and Technology,2007.
[8] Chao H R,Wei F,Zhang L L,et al. Isolation, Identification and Biodegradation Characteristics of a Novel Chlorobenzene-degrading Bacterial Strain. Environmental Science & Technology, 2011, 34(2): 6-11.
[9] Tang Y B,Wang X CH,Chen F Y,et al. Research on isolation and identification of a fluorene-degrading strain and its broad-spectrum property for degradation of polycyclic aromatic hydrocarbons. Chinese Journal of Environmental Engineering, 2011, 05(2): 467-471.
[10] Crispin H.Pierce.Yill chen,Russell L Dilis,etal.Toluene metabolites as biological indicators ofexposure,Toxicology Letters,2002,129:65-76
[11] Jin Y M,Chen J M. Applications of biofiltration in air pollution control processes. Techniques and Equipment for Environmental Pollution Control, 2001, 2(3): 76-80.
[12] Liao Q,Tian X,Zhu X,et al. Purifying waste gas containing low concentration toluene in trickling biofilter with ceramic spheres. Journal of Chemical Industry and Engineering(China), 2003, 54(12): 1774-1778.
[13] Jin-Ying X,Hong-Ying H U,Hong-Bo Zhu,et al. Comparison of degradation properties of two strains of toluene efficient degradation bacteria. China Environmental Science, 2005, 25(z1): 102-105.
[14] 尤志杰.微量液—液萃取气相色谱法测定水中的苯系物. 环境与开发, 1999(1):50. You Z J.Determination of benzene series in water with microliquid-liquid extraction cas Chromatography.Environment and Exploitation,1999(1):50.
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