技术与方法 |
|
|
|
|
利用核糖体展示技术筛选口蹄疫病毒 单链抗体基因 |
代鹏, 周广青, 林密, 冯霞, 马军武, 张晓霞 |
中国农业科学院兰州兽医研究所 家畜疫病病原生物学国家重点实验室 国家口蹄疫参考实验室 甘肃省生物检测工程技术研究中心 兰州 730046 |
|
Selection of scFv Genes of Foot-and-Mouth Disease Virus Using Ribosome Display |
DAI Peng, ZHOU Guang-qing, LIN Mi, FENG Xia, MA Jun-wu, ZHANG Xiao-xia |
State Key Laboratory of Veterinary Etiological Biology, National Foot and Mouth Disease Reference Laboratory, Gansu Provincial Engineering and Technique Research Centre on Biological Detection, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China |
引用本文:
代鹏, 周广青, 林密, 冯霞, 马军武, 张晓霞. 利用核糖体展示技术筛选口蹄疫病毒 单链抗体基因[J]. 中国生物工程杂志, 2012, 32(07): 95-101.
DAI Peng, ZHOU Guang-qing, LIN Mi, FENG Xia, MA Jun-wu, ZHANG Xiao-xia. Selection of scFv Genes of Foot-and-Mouth Disease Virus Using Ribosome Display. China Biotechnology, 2012, 32(07): 95-101.
链接本文:
https://manu60.magtech.com.cn/biotech/CN/
或
https://manu60.magtech.com.cn/biotech/CN/Y2012/V32/I07/95
|
[1] Yan X, Xu Z. Ribosome-display technology: applications for directed evolution of functional proteins. Drug Discov Today, 2006, 11(19-20): 911-916. [2] He M, Liu H, Turner M, et al. Detection of protein-protein interactions by ribosome display and protein in situ immobilization. N Biotechnol, 2009, 26(6): 277-281. [3] Lipovsek D, Plückthun A. In-vitro protein evolution by ribosome display and mRNA display. J Immunol Methods, 2004, 290(1-2): 51-67. [4] Zhao X L, Chen W Q, Yang Z H, et al. Selection and affinity maturation of human antibodies against rabies virus from a scFv gene library using ribosome display. J Biotechnol, 2009, 144(4): 253-258. [5] Hagemeyer C E, von Zur Muhlen C, von Elverfeldt D, et al. Single-chain antibodies as diagnostic tools and therapeutic agents. Thromb Haemost, 2009, 101(6): 1012-1019. [6] Elbakri A, Nelson P N, Abu Odeh R O. The state of antibody therapy. Hum Immunol, 2010, 71(12): 1243-1250. [7] Klein J. Understanding the molecular epidemiology of foot-and-mouth-disease virus. Infect Genet Evol, 2009, 9(2):153-161. [8] 杨双, 马军武, 周广青, 等. 核糖体展示口蹄疫单链抗体库的构建. 中国生物工程杂志, 2011, 31(7): 98-103. Yang S, Ma J W, Zhou G Q, et al. Construction of ribosome display single chain antibody library of foot-and-mouth disease. China Biotechnology, 2011, 31(7): 98-103. [9] 王春奕, 马军武, 伏小平. 口蹄疫病毒的纯化及鉴定. 中国兽医科技, 2004, 34(7): 66-69. Wang C Y, Ma J W, Fu X P. Purification and examination of foot-and-mouth disease virus. Chinese Journal of Veterinary Science and Technology, 2004, 34(7): 66-69. [10] Sergeeva A, Kolonin M G, Molldrem J J, et al. Display technologies: Application for the discovery of drug and gene delivery agents. Adv Drug Deliv Rev, 2006, 58(15): 1622-1654. [11] Hanes J, Jermutus L, Weber-Bornhauser S, et al. Ribosome display efficiently selects and evolves high-affinity antibodies in vitro from immune libraries. Proc Natl Acad Sci USA, 1998, 24, 95(24): 14130-14135. [12] Hanes J, Schaffitzel C, Knappik A, et al. Picomolar affinity antibodies from a fully synthetic na?ve library selected and evolved by ribosome display. Nat Biotechnol, 2000, 18(12): 1287-1292. [13] Jackson T, King A M, Stuart D I, et al. Structure and receptor binding. Virus Res, 2003, 91(1):33-46. |
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
|
Shared |
|
|
|
|
|
Discussed |
|
|
|
|