海洋环境来源的淀粉酶AmyP对生玉米 淀粉的降解特性

中国生物工程杂志

2012, Vol. 32

Issue (07): 79-83

研究报告

海洋环境来源的淀粉酶AmyP对生玉米淀粉的降解特性

彭惠, 雷寅, 刘源涛, 汪颖

安徽大学生命科学学院安徽省微生物与生物催化工程技术研究中心合肥 230039

Degradation of Raw Corn Starch by an α-Amylase (AmyP) from Marine Environment

PENG Hui, LEI Yin, LIU Yuan-tao, WANG Ying

School of Life Sciences, Anhui University, Engineering Technology Research Center of Microorganisms and Biocatalysis, Hefei 230039, China

全文: PDF(531 KB) HTML

摘要： 来自海洋宏基因组文库的α-淀粉酶(AmyP)属于最新建立的糖苷水解酶亚家族GH13_37。AmyP是一个生淀粉降解酶,能有效降解玉米生淀粉。在最适反应条件pH 7.5和40℃下,生玉米淀粉的比活达到(39.6±1.4)U/mg。酶解反应动力学显示AmyP可以非常快速的降解生玉米淀粉。对1%的生玉米淀粉降解仅需要30min;4%和8%的生玉米淀粉只需3h。DTT可以显著提高AmyP对生玉米淀粉的降解活性,1% DTT促使活性增加1倍。根据电镜观察和产物分析,认为AmyP是以内腐蚀的模式降解生玉米淀粉颗粒,释放出葡萄糖、麦芽糖和麦芽三糖作为终产物。

关键词： α-淀粉酶; 生玉米淀粉; 糖苷酶家族GH13_37; 酶解

Abstract: The α-amylase (AmyP) from a marine metagenomic library belongs to the recently classified glycoside hydrolase subfamily GH13_37. AmyP is raw starch degrading enzyme, exhibiting a remarkable ability to digest raw corn starch. The specific activity of raw corn starch was reached (39.6 ± 1.4)U/mg under the optimum pH 7.5 and temperature 40℃. The hydrolysis curve showed that AmyP could hydrolyze raw corn starch at a very high speed. The final hydrolysis degrees were obtained in 30min for 1% raw corn starch and 3h for 4% and 8% concentration. The enzyme’s activity was greatly increased in the presence of DTT. 1% DTT led to a twofold-enhanced activity. The results of scanning electron microscopy and thin-layer chromatography show that AmyP attacks sites on raw corn starch granules with a mode of endo-corrosion, and releases glucose, maltose and maltotriose as end products.

Key words: α-Amylase Raw corn starch Glycoside hydrolase subfamily GH13_37 Hydrolysis

收稿日期: 2012-05-05 出版日期: 2012-07-25

ZTFLH:

Q556

基金资助: 安徽省自然科学基金(11040606M65);安徽省高等学校省级自然科学研究重点项目(KJ2012A017)资助项目

通讯作者: 彭惠 E-mail: pph0259@126.com

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引用本文:

彭惠, 雷寅, 刘源涛, 汪颖. 海洋环境来源的淀粉酶AmyP对生玉米淀粉的降解特性[J]. 中国生物工程杂志, 2012, 32(07): 79-83.

PENG Hui, LEI Yin, LIU Yuan-tao, WANG Ying. Degradation of Raw Corn Starch by an α-Amylase (AmyP) from Marine Environment. China Biotechnology, 2012, 32(07): 79-83.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/ 或 https://manu60.magtech.com.cn/biotech/CN/Y2012/V32/I07/79

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