响应面法优化木薯淀粉发酵生产单细胞油脂工艺

中国生物工程杂志

2012, Vol. 32

Issue (07): 66-72

研究报告

响应面法优化木薯淀粉发酵生产单细胞油脂工艺

艾佐佐¹, 颜日明¹, 袁锦云¹, 张志斌¹, 朱笃^1,2

1. 江西师范大学生命科学学院江西省亚热带植物资源保护与利用重点实验室南昌 330022;
2. 宜春学院江西省天然药物活性成分研究重点实验室宜春 336000

Optimization of Single Cell Oil Produced from Cassava Starch by Response Surface Methodology

AI Zuo-zuo¹, YAN Ri-ming¹, YUAN Jin-yun¹, ZHANG Zhi-bin¹, ZHU Du^1,2

1. Key Laboratory of Protection and Utilization of Subtropic Plant Resources of Jiangxi Province, Jiangxi Normal University, Nanchang 330022, China;
2. Key Laboratory for Research on Active Ingredients in Natural Medicine of Jiangxi Province, Yichun University, Yichun 336000, China

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摘要： 采用响应面分析法对发酵性丝孢酵母菌株以木薯淀粉水解液为原料合成微生物油脂的培养条件进行优化。首先利用Plackett-Burman试验设计确定影响油脂产量的主要因素,在此基础上再利用Box-Behnken试验设计及响应面分析法对其进行条件优化。结果表明,发酵温度、C/N、pH对油脂产量具有显著影响,产油脂的最佳发酵条件为:发酵温度 28.78℃、C/N 126.18、pH 6.69,油脂产量达到14.88g/L,比优化前提高了28.6%。同时,气相色谱分析表明,微生物油脂脂肪酸组成成分主要包括棕榈酸、硬脂酸、油酸、亚油酸酯等,是优良的生物柴油制备原料。

关键词： 木薯淀粉; 响应面分析法; 单细胞油脂; 条件优化; 发酵性丝孢酵母

Abstract: Response surface methodology was applied to optimize the Trichosporon fermentans fermentation conditions for microbial lipids produced from hydrolysate of cassava starch. The Plackeet-Burman design was adopted to sort the important factors influencing the lipids yield, and the conditions of lipids production was further optimized by using Box-Behnken design and response surface methodology. The results showed that the temperature, C/N and pH could influence on the lipid yield significantly. The optimized conditions for lipid production were temperature of 28.79℃, C/N of 126.18 and pH of 6.69, and the lipids yield of 14.88g/L which was 28.6% higher than control. Moreover, gas chromatography analysis revealed that the microbial lipid from Trichosporon fermentans mainly included palmitic acid, stearic acid, oleic acid and linoleic acid and it was suggested to be used as an excellent feedstock for biodiesel production.

Key words: Cassava starch Response surface methodology Microbial lipids Condition optimization Trichosporon fermentans

收稿日期: 2012-03-06 出版日期: 2012-07-25

ZTFLH:

Q815

基金资助: 国家"863"计划(2012AA021205);江西省主要学科学术与技术带头人培养计划(060002)资助项目

通讯作者: 朱笃 E-mail: zhudu12@163.com

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引用本文:

艾佐佐, 颜日明, 袁锦云, 张志斌, 朱笃. 响应面法优化木薯淀粉发酵生产单细胞油脂工艺[J]. 中国生物工程杂志, 2012, 32(07): 66-72.

AI Zuo-zuo, YAN Ri-ming, YUAN Jin-yun, ZHANG Zhi-bin, ZHU Du. Optimization of Single Cell Oil Produced from Cassava Starch by Response Surface Methodology. China Biotechnology, 2012, 32(07): 66-72.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/ 或 https://manu60.magtech.com.cn/biotech/CN/Y2012/V32/I07/66

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