绵羊羊膜上皮细胞治疗兔桡骨缺损模型的研究

中国生物工程杂志

2012, Vol. 32

Issue (07): 31-36

研究报告

绵羊羊膜上皮细胞治疗兔桡骨缺损模型的研究

朱雪敏, 刘俊平, 杨银凤, 王秀梅, 米焱, 李海军, 李砚, 孟庆刚, 曹贵方

内蒙古农业大学动物组织胚胎与发育生物学研究室呼和浩特 010018

The Study of Therapy Efficacy Applied Sheep Amnion Epithelial Cells in Rabbit Radius Defected Models

ZHU Xue-min, LIU Jun-ping, YANG Yin-feng, WANG Xiu-mei, MI Yan, LI Hai-jun, LI Yan, MENG Qing-gang, CAO Gui-fang

Laboratory of Animal Embryo and Developmental Biology, Inner Mongolia Agricultural University, Huhhot 010018, China

全文: PDF(1182 KB) HTML

摘要： 为了确定绵羊羊膜上皮细胞在体内向骨组织的分化能力,实验在分离培养绵羊羊膜上皮细胞并对其进行干细胞特性的鉴定的基础上,制作新西兰大白兔桡骨13mm骨缺损模型,随机分组对其进行注射绵羊羊膜上皮细胞实验。高剂量组:移植细胞5×10⁷个;低剂量组:移植细胞5×10⁶个;对照组:生理盐水。细胞移植后2、4、8周拍摄X光片观察骨缺损部位的缺损修复情况;相应时段取骨缺损部位新生骨进行组织学观察:分析骨小梁生成数量和骨的改建时期。实验结果显示,高剂量实验组在移入细胞第8周,骨缺损完全修复,且同期高剂量组新骨生成的数量和质量明显高于低剂量组,低剂量组优于对照组。由此可见,绵羊羊膜上皮细胞不仅可以在不同种动物间进行移植,而且对骨缺损有良好的修复能力。

关键词： 绵羊; 羊膜上皮细胞; 干细胞特性; 桡骨缺损

Abstract: To explore the potentials differentiation of sheep amnion epithelial cells into bone in vitro, the sheep amnion epithelial cells of stem cells properties were injected into New Zealand rabbit radius defected models and then observed the radius restoration. The sheep amnion epithelial cells were cultured and made morphological identifications. The rabbit’s left 13-centimetre long radius defected models were artificially divided into high dose group, low dose group and control group randomly. Sheep amnion epithelial cells with the dense of 5×10⁷ and 5×10⁶ in 5ml saline and 5ml saline were injected into high dose group of rabbits, low dose group of rabbits and control group of rabbits respectively. Took the models for X-ray in 2 weeks, 4 weeks and 8 weeks after the sheep amnion epithelial cells transplantations respectively and observed the radius restorations. At the mean time, the radius defected areas were observed by histological methods, and the trabecular bones generating quantities and the bone remodeling time were analysised. Defected radiuses in high dose group of models were restored completely at 8 weeks after the sheep amnion epithelial cells transplantations. Meanwhile, the quality and quantities of trabecular bones in high dose group were better and higher than in the low dose group and it was the same situation between the low dose group and the control group. Thus it can be seen that the sheep amnion epithelial cells can not only be transplanted among various species of animal, but also are effective for defective bone restoration.

Key words: Sheep Amnion epithelial cells Stem cells properties Radius defection

收稿日期: 2012-03-09 出版日期: 2012-07-25

ZTFLH:

基金资助: 国家"863"计划资助项目(2008AA101005)

通讯作者: 刘俊平, 曹贵方 E-mail: guifangcao@126.com; liujp8539@126.com

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引用本文:

朱雪敏, 刘俊平, 杨银凤, 王秀梅, 米焱, 李海军, 李砚, 孟庆刚, 曹贵方. 绵羊羊膜上皮细胞治疗兔桡骨缺损模型的研究[J]. 中国生物工程杂志, 2012, 32(07): 31-36.

ZHU Xue-min, LIU Jun-ping, YANG Yin-feng, WANG Xiu-mei, MI Yan, LI Hai-jun, LI Yan, MENG Qing-gang, CAO Gui-fang. The Study of Therapy Efficacy Applied Sheep Amnion Epithelial Cells in Rabbit Radius Defected Models. China Biotechnology, 2012, 32(07): 31-36.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/ 或 https://manu60.magtech.com.cn/biotech/CN/Y2012/V32/I07/31

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