Aurora-A高表达对食管癌细胞侵袭和 MMP-9表达的影响

中国生物工程杂志

2012, Vol. 32

Issue (07): 26-30

研究报告

Aurora-A高表达对食管癌细胞侵袭和 MMP-9表达的影响

王晓霞^1,2, 路娜¹, 刘志荣¹, 解军¹, 王惠珍¹, 牛勃¹, 裴毅²

1. 山西医科大学生物化学与分子生物学教研室太原 030001;
2. 山西医学科学院/山西大医院太原 030032

Effect of Aurora-A Overexpression on Invasion and MMP-9 Expression of Esophageal Carcinoma Cells

WANG Xiao-xia^1,2, LU Na¹, LIU Zhi-rong¹, XIE Jun¹, WANG Hui-zhen¹, NIU Bo¹, PEI Yi²

1. Department of Biochemistry and Molecular Biology, Shanxi Medical University, Taiyuan 030001, China;
2. Shanxi Academy of Medical Sciences, Shanxi Dayi Hospital, Taiyuan 030032, China

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摘要： 目的:建立Aurora-A高表达的稳定细胞系,并探讨Aurora-A高表达对食管癌细胞侵袭和MMP-9蛋白表达与活性的影响。方法:利用脂质体法稳定转染Aurora-A表达质粒,通过Western blot和RT-PCR鉴定Aurora-A高表达的稳定细胞系。利用细胞体外侵袭实验观察细胞的侵袭能力,并通过Western blot、ELISA和明胶酶谱的方法观察MMP-9的蛋白表达水平和活性。结果:建立了Aurora-A高表达的稳定细胞系,且Aurora-A高表达能增加食管癌细胞的体外侵袭能力,并提高了MMP-9的蛋白表达水平和活性。结论:初步阐明了Aurora-A促进食管癌细胞侵袭的作用以及分子机制,为Aurora-A可能成为治疗食管癌的有效靶点提供了依据。

关键词： Aurora-A; 侵袭; MMP-9

Abstract: Objective:To establish Aurora-A overexpressing transfectant clones and investigate its effect on invasiveness as well as protein expression and activity of MMP-9 in esophageal carcinoma cells. Methods:The Aurora-A expressing plasmid was transfected using Lipofectamine^TM 2000 and the identification of transfected clones was confirmed by Western blot analysis and RT-PCR. The invasive properties of cells were examined by an in vitro Boyden chamber invasion assay. Level of MMP-9 protein expression and activity were measured with Western blot and ELISA as well as gelatin zymography analysis. Results:Aurora-A overexpressing transfectant clone was established, which increased cell invasion as well as protein expression and activity of MMP-9. Conclusion:The elucidation the molecular mechanism for the promotion of invasion in esophageal carcinoma by Aurora-A may lead to the development of new target for effective therapy.

Key words: Aurora-A Invasion MMP-9

收稿日期: 2012-01-31 出版日期: 2012-07-25

ZTFLH:

R734.2

基金资助: 国家自然科学基金(30973401);山西省自然科学基金(2009011052-1)资助项目

通讯作者: 王晓霞 E-mail: wxiaoxia99007@yahoo.com.cn

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引用本文:

王晓霞, 路娜, 刘志荣, 解军, 王惠珍, 牛勃, 裴毅. Aurora-A高表达对食管癌细胞侵袭和 MMP-9表达的影响[J]. 中国生物工程杂志, 2012, 32(07): 26-30.

WANG Xiao-xia, LU Na, LIU Zhi-rong, XIE Jun, WANG Hui-zhen, NIU Bo, PEI Yi. Effect of Aurora-A Overexpression on Invasion and MMP-9 Expression of Esophageal Carcinoma Cells. China Biotechnology, 2012, 32(07): 26-30.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/ 或 https://manu60.magtech.com.cn/biotech/CN/Y2012/V32/I07/26

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