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tRNase Z核酸内切酶的研究进展
tRNase Z’s Research Progress
tRNase Z是一种核酸内切酶,许多细菌、大多数真核生物以及所有的古细菌的tRNA3'末端加工过程都是由核酸内切酶tRNase Z催化的。tRNase Z能催化缺乏CCA的tRNA前体生成末尾带有核苷酸识别的3'-OH和5'磷酸尾巴的成熟tRNA。这对于CCA序列的添加、tRNA的氨酰化和蛋白质的合成十分重要。tRNase Z属于metallo-β-lactamases(MBL)超家族,存在短(tRNase ZS) 和长(tRNase ZL)两种形式,具有tRNA 3'末端加工、引导定位蛋白、加工rRNA、与Rex2P的相互作用、调节细胞分化与分裂等功能。预期对tRNaseZ的功能和属性不断深入研究将会对AIDS和前列腺癌的治疗具有潜在和实际的推动作用。
tRNase Z is an endonuclease that catalyzes tRNA 3'-end processing in many bacteria, most eukaryotes and all archaea. tRNase Z generates a mature tRNA ending with the 3'-OH of the discriminator nucleotide and a trailer sequence with a 5'-phosphate from CCA-less tRNA precursors, which is absolutely essential for the addition of the CCA sequence, tRNA aminoacylation and protein synthesis. tRNase Z enzymes belong to the superfamily of metallo-β-lactamases. tRNase Z exists the short (tRNase ZS) and long (tRNase ZL) forms. tRNase Z has many functions, such as RNA 3'-end processing, guiding the positioning of protein, rRNA-processing, complementation with the REX2 gene, regulation of cell proliferation and differentiation etc. Further analysis of the function and properties of tRNase Z will play a potential and actual role in the treatment of AIDS and prostate cancer in the coming years.
tRNase Z / metallo-&beta / -lactamases(MBL)超家族 / 3&rsquo / -CCA / ELAC2 {{custom_keyword}} /
tRNase Z / Metallo-β-lactamases(MBL)superfamily / 3'-CCA / ELAC2 {{custom_keyword}} /
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国家自然科学基金(30970346,31172076)、中央高校基本科研业务费专项资金(GK201001004)、陕西省自然科学基金(2007C101)资助项目
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