幽门螺杆菌Ure B串联融合表位的表面呈现表达

中国生物工程杂志

2011, Vol. 31

Issue (8): 35-39

研究报告

幽门螺杆菌Ure B串联融合表位的表面呈现表达

封小燕^1,2, 王艳春², 李平超², 陶好霞², 王芃², 袁盛凌², 王令春², 王普¹, 刘纯杰²

1. 浙江工业大学药学院杭州 310032;
2. 军事医学科学院生物工程研究所病原微生物与生物安全国家重点实验室北京 100071

Surface Display Expression of Helicobacter pylori Ure B Fusion Epitope

FENG Xiao-yan^1,2, WANG Yang-chun², LI Ping-chao², TAO Hao-xia², WANG Peng², YUAN Sheng-ling², WANG Ling-chun², WANG Pu¹, LIU Chun-jie²

1. College of Pharmaceutical Science,Zhejiang University of Technology,Hangzhou 310032,China;
2. Beijing Institute of Biotechnology,State Key Laboratory of Pathogen and Biosecurity,Beijing 100071,China

全文: PDF(674 KB) HTML

摘要：

目的:应用表达载体pHIE3N表面呈现表达幽门螺杆菌Ure B串联融合表位。方法:通过引入多克隆位点的方法改造表达呈现表达载体pHIE11,并用改造后的质粒表达幽门螺杆菌Ure B串联融合表位,用SDS-PAGE、Western blot和全菌ELISA检测所得到的重组菌。结果:改造的质粒插入序列正确,能够特异性呈现表达Ure B串联融合表位,表达产物的分子量约为60kDa,与预期大小一致。全菌ELISA结果表明Ure B串联融合表位表达于菌体表面。结论:pHIE3N载体能够表面展示呈现Ure B串联融合表位,构建的重组菌为幽门螺杆菌候选疫苗的研发奠定了基础。

关键词： 表面展示; pHIE3N; ure B串联表位

Abstract:

Objective:To achieve surface display of H.pylori ure B fusion epitope by vector pHIE3N. Methods:Multiple cloning site was introduced into pHIE3N in order to express the H.pylori ure B fusion epitope protein. The recombinant strain was confirmed by SDS-PAGE,Western blot and ELISA. Result:The inserted sequence of the reconstructed plasmid was correct. The molecular weight of the fused Ure B protein was about 60 kDa which had proved by SDS-PAGE and Western blot analysis,ELISA results showed that the Ure B fusion epitope expressed on cell surfaces. Conclusion:The Ure B fusion epitope was successfully expressed on the sruface of E.coli DH5α using pHIE3N surface display vector. This result will contribute to the vaccine research of H.pylori.

Key words: Surface display pHIE3N ure B fusion epitope

收稿日期: 2011-03-22 出版日期: 2011-08-25

ZTFLH:

Q819

基金资助:

"十一五"国家科技支撑计划项目(2008BAI66B03)、 "十一五"国家艾滋病和病毒性肝炎等重大传染病防治重大专项(2008ZX1004-015)资助项目

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引用本文:

封小燕, 王艳春, 李平超, 陶好霞, 王芃, 袁盛凌, 王令春, 王普, 刘纯杰. 幽门螺杆菌Ure B串联融合表位的表面呈现表达[J]. 中国生物工程杂志, 2011, 31(8): 35-39.

FENG Xiao-yan, WANG Yang-chun, LI Ping-chao, TAO Hao-xia, WANG Peng, YUAN Sheng-ling, WANG Ling-chun, WANG Pu, LIU Chun-jie. Surface Display Expression of Helicobacter pylori Ure B Fusion Epitope. China Biotechnology, 2011, 31(8): 35-39.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/ 或 https://manu60.magtech.com.cn/biotech/CN/Y2011/V31/I8/35

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