产喜树碱喜树内生真菌的筛选及喜树内生真菌的SRAP分析

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中国生物工程杂志 ›› 2011, Vol. 31 ›› Issue (7) : 60-64.

研究报告

产喜树碱喜树内生真菌的筛选及喜树内生真菌的SRAP分析

李霞¹, 刘佳佳¹, 陈建华², 栾明宝², 殷珍珍¹, 杨栋梁¹

作者信息 +

Screening of Camptothecin Production and SRAP Analysis of Endophytic Fungi from Camptotheca acuminata Decne

LI Xia¹, LIU Jia-jia¹, CHEN Jian-hua², LUAN Ming-bao², YIN Zhen-zhen¹, YANG Dong-liang¹

Author information +

文章历史 +

摘要

生活在宿主植物里的内生真菌是很重要的药用资源。喜树是中国的传统药用植物。从喜树植物中分离得到了大约50种菌株,其中一株产喜树碱的菌株通过形态学鉴定为青霉属,这是首次在喜树植物中发现产喜树碱的青霉属菌株。为研究简单序列重复相关序列扩增多态性(SRAP)方法在喜树内生真菌中应用的可行性,选择了十株喜树内生真菌进行SRAP多态性分析。SRAP引物共扩增出1 295条带,而这些菌株也被分为三大类。这些结果表明,SRAP研究喜树内生真菌具有高效性,是讨论喜树内生真菌的遗传多样性的有效方法。

Abstract

Endophytic fungi, lived in stems and leaves of host plants, were supposed to be effective or novel sources for therapeutic compounds. Camptotheca acuminata Decne is a traditional medical plant in China. About 50 endophytic fungi were isolated from barks of C. acuminata. A new fungus producing camptothecin (CPT) was identified as a Penicillium based on its morphological features. Ten endophytic fungi of C. acuminata were chosen to be amplified using molecular maker sequence related amplified polymorphism (SRAP). The combinations of SRAP primers turned out to be polymorphic and a total of 1 295 polymorphic bands were obtained. These strains were grouped into 3 main clusters and revealed that SRAP could isolate the strains of C. acuminata efficiently. It also provided evidence for discussing the genetic diversities of species in C. acuminata with SRAP molecular method.

导出引用

李霞, 刘佳佳, 陈建华, 栾明宝, 殷珍珍, 杨栋梁. 产喜树碱喜树内生真菌的筛选及喜树内生真菌的SRAP分析[J]. 中国生物工程杂志, 2011, 31(7): 60-64

LI Xia, LIU Jia-jia, CHEN Jian-hua, LUAN Ming-bao, YIN Zhen-zhen, YANG Dong-liang. Screening of Camptothecin Production and SRAP Analysis of Endophytic Fungi from Camptotheca acuminata Decne[J]. China Biotechnology, 2011, 31(7): 60-64

中图分类号： Q75

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