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中国生物工程杂志

CHINA BIOTECHNOLOGY
中国生物工程杂志  2011, Vol. 31 Issue (03): 101-106    
综述     
绵羊腺病毒287作为基因传递载体的应用前景
谷元兴, 王猛, 刘文倩, 张杰, 刘永生
中国农业科学院兰州兽医研究所 兰州 730046
The Application Prospects of Ovine Adenovirus287 Vector
GU Yuan-xing, WANG Meng, LIU Wen-qian, ZHANG Jie, LIU Yong-sheng
Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046,China
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摘要:

绵羊腺病毒287(OAdV287) 具有独特的基因组结构,全长29 576bp。基因组最大的特点是有很高的A/T含量(66.4%)和缺少典型的E1区,以及含有一段较长的冗余片段。已识别出其基因组中有3个外源基因插入区,理论上最多可容纳6.3kb的外源DNA。OAdV287的纤维和五邻体结构结构独特,衣壳蛋白上缺少可识别的整合蛋白结合域。OAdV287能感染一系列人和动物的细胞系,但是不能在非绵羊源细胞内成功复制。OAdV287载体可以避免人腺病毒的免疫干扰,具有很高的转染效率。目前,OAdV287已成为研究最热的动物源腺病毒载体之一。

关键词: 绵羊腺病毒基因组结构基因载体应用    
Abstract:

Ovine adenovirus 287 has a unique genome organization with a length of 29 576bp. The genome is characterized distinctively by a high A/T content (66.4%), the lack of a typical El region, and a long redundant sequence. Three nonessential regions of the viral genome, where 6.3kb foreign genes can be inserted theoretically, have been identified. OAdV287 also has unique fiber and penton, where lacks the integrin-binding domain in penton or any other capsid protein. OAdV287 can infect a wide range of human and animal cell lines, but failing to replicate in non-ovine cell lines. In addition, OAdV287 vector can circumvent the pre-existing human adenoviruses immunity, and so have an efficient transfection. Currently, OAdV287 has become one of the research focuses among the animal adenoviruses vectors.

Key words: Ovine adenovirus    Genome structure    Gene vector    Applications
收稿日期: 2010-10-19 出版日期: 2011-04-01
ZTFLH:  Q782  
基金资助:

国家"转基因生物新品种培育"重大专项资助项目(2009ZX08007-006B)

通讯作者: 谷元兴     E-mail: jinguyuan2010@yahoo.cn;liuyongshengvip@sina.com.cn
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引用本文:

谷元兴, 王猛, 刘文倩, 张杰, 刘永生. 绵羊腺病毒287作为基因传递载体的应用前景[J]. 中国生物工程杂志, 2011, 31(03): 101-106.

GU Yuan-xing, WANG Meng, LIU Wen-qian, ZHANG Jie, LIU Yong-sheng. The Application Prospects of Ovine Adenovirus287 Vector. China Biotechnology, 2011, 31(03): 101-106.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/        https://manu60.magtech.com.cn/biotech/CN/Y2011/V31/I03/101

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