大孔树脂对三孢布拉霉菌产番茄红素的吸附性能研究

中国生物工程杂志

2010, Vol. 30

Issue (04): 71-76

研究报告

大孔树脂对三孢布拉霉菌产番茄红素的吸附性能研究

李干禄,吴启赐,武振军,石楠,万红贵^*

南京工业大学生物与制药工程学院南京 210009

Study on Adsorption Properties of Lycopene from the Broth of Blakeslea trispora by Macroporous Resin

LI Gan-lu,WU Qi-ci,WU Zhen-jun,SHI Nan,WAN Hong-gui

Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing 210009,China

全文: PDF(1205 KB) HTML

摘要：

为利用大孔吸附树脂分离纯化三孢布拉霉菌发酵液番茄红素，考察了4种大孔树脂(HP20, HP2MG, SP825, SP207)对番茄红素的吸附解吸特性，筛选出最佳树脂。结果表明，HP20大孔树脂对番茄红素具有较好的吸附解吸性能，且吸附迅速，能在1h内达到吸附平衡。用含55%和60%异丙醇的丙酮不连续梯度洗脱，回收率可达89.55%，重结晶后可获得纯度95%以上的番茄红素，经红外、质谱、核磁等鉴定为天然型番茄红素。该方法简单，可操作性强，极具工业应用价值。

关键词： 三孢布拉霉菌; 番茄红素; 大孔树脂; 不连续梯度洗脱

Abstract:

The potential of macroporous resin for separation and purification of lycopene produced by B.trispora were investigated. The adsorptiondesorption properties for lycopene of the resins such as HP20, HP2MG, SP825 and SP207 were studied. The optimal resin for lycopene was screened and selected. The results expressed that HP20 resin had a better adsorption-desorption property for lycopene. It could quickly reach an adsorption balance in one hour. Purification of lycopene using packed bed column by HP20 resin was carried out using step gradient of 55% isopropyl alcohol in acetone followed by 65% isopropyl alcohol in acetone. A 89.55% recovery of lycopene was detected by HPLC. After further recrystallisation the purity of lycopene was above 95% and the structure was confirmed by IR, MS and 1H-NMR. The whole process was simple, easy to operate, with a good industrial application value.

Key words: Blakeslea trispora Lycopene Macroporous resin Step gradient elution

收稿日期: 2009-12-16 出版日期: 2010-04-29

通讯作者: 万红贵 E-mail: hg_wan@sina.com

	服务
	把本文推荐给朋友
	加入引用管理器
	E-mail Alert
	RSS
	作者相关文章
	李干禄
	吴启赐
	武振军
	石楠
	万红贵

引用本文:

李干禄吴启赐武振军石楠万红贵. 大孔树脂对三孢布拉霉菌产番茄红素的吸附性能研究[J]. 中国生物工程杂志, 2010, 30(04): 71-76.

LI An-Lu, TUN Qi-Ci, WU Zhen-Jun, DAN Nan, MO Gong-Gui. Study on Adsorption Properties of Lycopene from the Broth of Blakeslea trispora by Macroporous Resin. China Biotechnology, 2010, 30(04): 71-76.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/ 或 https://manu60.magtech.com.cn/biotech/CN/Y2010/V30/I04/71

［1］ Clinton S K. Lycopene: chemistry, biology, and implications for human health and disease. Nutr Rev, 1998, 56 (2): 35.
［2］ Bramley P M. Is lycopene beneficial to human health?. Phytochemistry, 2000, 54 (3): 23.
［3］ Pohar K S, Gong M C, Bahnson R, et al. Tomatoes, lycopene and prostate cancer: a clinician’s guide for counseling those at risk for prostate cancer. World J Urol, 2003, 21 (1): 9.
［4］汪茂田,谢培山,王忠东,等. 天然有机化合物提取分离与结构鉴定. 北京:化学工业出版社, 2004, 8: 115122. Wang M T, Xie P S, Wang Z D, et al. Natural Products Isolation and Structure Determination. Beijing: Chemical Industry Press, 2004.8: 115122.
［5］左榘,粟方星,朱常英,等. 聚丙烯酸凝胶溶胀行为的研究.离子与交换吸附, 1998, 15(1): 2329. Zuo J, Li F X, Zhu C Y,et al. Ion Exchange and Adsorption, 1998, 15(1): 2329.
［6］李芳,孔令明,冯作山. 番茄红素结晶溶剂选择的初步研究.食品工业科技, 2006, 27(12): 101103. Li F, Kong L M,Feng Z S. Science and Technology of Food Industry, 2006, 27(12): 101103.
［7］惠伯棣. 类胡萝卜素化学及生物化学.北京：中国轻工业出版社. 2005,1:179185. Hui B D. Beijing:China Light Industry Press, 2005.1:179185.［8］ Barrie Tan. Analytical and preparative chromatography of tomato paste carotenoids. Journal of Food Science,1988, 53(3):954959.

[1]	张映曈, 陈海琴, 宋元达, 张灏, 陈永泉, 陈卫. *卷枝毛霉pyrG基因缺陷突变株的诱变筛选与鉴定*[J]. 中国生物工程杂志, 2016, 36(3): 38-42.
[2]	李从镇, 毛宁. 大孔吸附树脂分离纯化培养基残基中虫草素[J]. 中国生物工程杂志, 2014, 34(1): 90-94.
[3]	王卫, 李忠海, 黎继烈, 曾泊全. 赤霉素发酵与大孔树脂吸附过程耦合工艺优化[J]. 中国生物工程杂志, 2012, 32(11): 70-74.
[4]	王卫, 李忠海, 黎继烈, 曾泊全. 赤霉素发酵与大孔树脂吸附过程耦合工艺优化[J]. 中国生物工程杂志, 2012, 32(11): 70-74.
[5]	林琼珊, 唐咸来, 李献, 蒋承建, 李俊芳, 申佩弘. *甲基营养菌 Methylobacterium* sp MB200 中crtI基因的克隆及表达**[J]. 中国生物工程杂志, 2012, 32(08): 56-61.
[6]	霍培, 季静, 王罡, 关春峰, 金超. 番茄红素β-环化酶基因的玉米转化及后代遗传分析[J]. 中国生物工程杂志, 2012, 32(07): 43-48.
[7]	魏浩, 陆兆新, 吕凤霞, 翟亚楠, 郭昊, 郝慧, 别小妹. Bacillus amyloliquefaciens ES-2发酵产抗菌脂肽消泡剂的筛选及脂肽的提取和纯化[J]. 中国生物工程杂志, 2011, 31(02): 85-90.
[8]	王敏杨慧高俊莲马荣才. 高产番茄红素链霉菌选育及摇瓶发酵研究[J]. 中国生物工程杂志, 2009, 29(12): 64-68.
[9]	万红贵,徐传学,佘勇. 三孢布拉霉生产番茄红素研究进展[J]. 中国生物工程杂志, 2009, 29(03): 105-109.
[10]	李晔,袁其朋. 产番茄红素基因工程菌的研究进展[J]. 中国生物工程杂志, 2006, 26(11): 81-86.
[11]	刘敏,李荣贵,杜桂彩,范海. 累积番茄红素的大肠杆菌工程菌及其培养条件的研究[J]. 中国生物工程杂志, 2006, 26(08): 47-51.

Viewed

Full text

Abstract

Cited

Shared

Discussed