细菌碱性果胶酶的酶学特性及其应用研究

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中国生物工程杂志 ›› 2010, Vol. 30 ›› Issue (04) : 125-130.

研究报告

细菌碱性果胶酶的酶学特性及其应用研究

陈丽娜,应向贤,薛群,汪钊^**

作者信息 +

Properties and Applications of Alkaline Pectinases from Bacteria

CHEN Li-na,YING Xiang-xian,XUE Qun,WANG Zhao

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文章历史 +

摘要

碱性果胶酶是最适作用pH值在碱性范围的果胶酶，由于其在碱性环境下活性较高的特点，在纺织和造纸等领域有良好的应用前景。从分子特性和催化特征等方面综述了源自野生菌和重组菌的细菌碱性果胶酶的酶学特性，并介绍了碱性果胶酶在棉织物精练、生物制浆和诱导植物抗病等领域的最新应用。

Abstract

Alkaline pectinases are a group of pectinolytic enzymes displaying optimal activities at alkaline pHs, which characteristic makes it feasible for them to be promising biocatalysts used in the textile and paper industry. The molecular characteristics and catalytic properties of native and recombinant alkaline pectinases from bacteria were summarized and discussed. The latest applications on bioscouring, biopulp making and induction of plant disease resistance were also stated.

导出引用

陈丽娜应向贤薛群汪钊. 细菌碱性果胶酶的酶学特性及其应用研究[J]. 中国生物工程杂志, 2010, 30(04): 125-130

CHEN Li-Na, YING Xiang-Xian, XUE Qun, HONG Zhao. Properties and Applications of Alkaline Pectinases from Bacteria[J]. China Biotechnology, 2010, 30(04): 125-130

参考文献

［1］ Bruhlmann F Purification and characterization of an extracellular pectate lyase from an Amycolata sp. Appl Environ Microbiol,1995,61:35803585.
［2］ Bruhlmann F, Keen N T. Cloning, sequence and expression of the pel gene from an Amycolata sp. Gene,1997,202:4551.
［3］ Bekri MYA, Desair J, Keijers V, et al. Azospirillum irakense produces a novel type of pectate lyase. J Bacteriol,1999,181:24402447.
［4］ Sawada K, Suzumatsu A, Kobayashi T. Molecular cloning and sequencing of the gene encoding an exopolygalacturonase of a Bacillus isolate and properties of its recombinant enzyme1. Biochimica et Biophysica Acta,2001,1568: 162170.
［5］ Zhai C, Cao J, Wang Y Cloning and expression of a pectate lyase gene from Bacillus alcalophillus NTT33. Enzyme Microb Technol,2003,33:173178.
［6］ Berensmeier S, Singh S A, Meens J, et al. Cloning of the pelA gene from Bacillus licheniformis 14A and biochemical characterization of recombinant, thermostable, highalkaline pectate lyase. Appl Microbiol Biotechnol, 2004,64:560567.
［7］刘连成,沈标.地衣芽孢杆菌碱性果胶酶基因PelA的克隆与原核表达.工业微生物,2008，38(1):2429. Liu L C, Shen B, et al. Industrial Microbiology,2008,38(1):2429.
［8］ KlugSantner B G, Schnitzhofer W, Vransk M, et al. Purification and characterization of a new bioscouring pectate lyase from Bacillus pumilus BK2. J Biotechnol,2006,121:390401.
［9］ Soriano M, Diaz P, Pastor F J. Pectate lyase C from Bacillus subtilis: a novel endocleaving enzyme with activity on highly methylated pectin. Microbiology,2006,152:617625.
［10］ Nasser W, Awade A C, Reverchon S, et al. Pectate lyase from Bacillus subtilis: molecular characterization of the gene, and properties of the cloned enzyme. FEBS Lett,1993,335:319326.
［11］ Nasser W, Chalet F, RobertBaudouy J. Purification and characterization of extracellular pectate lyase from Bacillus subtilis. Biochimie,1999,72:689695.
［12］ Zhuge B, Du G C, Shen W, et al. Efficient secretory expression of an alkaline pectate lyase gene from Bacillus subtilis in E. coli and the purification and characterization of the protein. Biotechnol Lett,2007,29:405410.
［13］ Kobayashi T, Hatada Y, Higaki N, et al. Enzymatic properties and deduced amino acid sequence of a highalkaline pectate lyase from an alkaliphilic Bacillus isolate. Biochim Biophys Acta,1999,1427:145154.
［14］ Ogawa A, Sawada K, Saito K, et al. A new highalkaline and highmolecularweight pectate lyase from a Bacillus Isolate: Enzymatic properties and cloning of the gene for the enzyme biosci.Biotechnol Biochem,2000,64(6):11331141.
［15］ Hatada Y, Saito K, Koike K, et al. Deduced aminoacid sequence and possible catalytic residues of a novel pectate lyase from an alkaliphilic strain of Bacillus. Eur J Biochem,2000, 267:22682275.
［16］ Hatada Y, Higaki N, Saito K, et al. Cloning and sequencing of a high alkaline pectate lyase gene from an alkaliphilic Bacillus isolate. Biosci Biotechnol Biochem,1999,63:9981005.
［17］ Kobayashi T, Hatada Y, Suzumatsu A, et al. Highly alkaline pectate lyase Pel4A from alkaliphilic Bacillus sp. strain P4N: its catalytic properties and deduced amino acid sequence. Extremophiles,2000,4:377383.
［18］ Hatada Y, Kobayashi T, Ito S. Enzymatic properties of the highly thermophilic and alkaline pectate lyase Pel4B from alkaliphilic Bacillus sp. strain P4N and the entire nucleotide and amino acid sequences. Extremophiles,2001,5:127133.
［19］ Takao M, Nakaniwa T, Yoshikawa K, et al. Purification and characterization of thermostable pectate lyase with protopectinase activity from thermophilic Bacillus sp. TS 47. Biosci Biotechnol Biochem,2000, 64:23602366.
［20］ Tamaru Y, Doi R H. Pectate lyase A, an enzymatic subunit of the Clostridium cellulovorans cellulosome. Proc Natl Acad Sci USA,2001, 98:41254129.
［21］ Favey S, Bourson C, Bertheau Y, et al. Purification of the acidic pectate lyase and nucleotide sequence of the corresponding gene (pelA) of Erwinia chrysanthemi strain 3937. J Gen Microbiol,1992,138:499508.
［22］ Tardy F, Nasser W, RobertBaudouy J, et al. Comparative analysis of the five major Erwinia chrysanthemi pectate lyases: enzyme characteristics and potential inhibitors. J Bacteriol,1997,179:25032511.
［23］ Brown I E, Mallen M H, Charnock S J, et al. Pectate lyase 10A from Pseudomonas cellulosa is a modular enzyme containing a family 2a carbohydratebinding module. Biochem J,2001, 355:155165.
［24］ Liao C H. Analysis of pectate lyases produced by soft rot bacteria associated with spoilage of vegetables. Appl Environ Microbiol,1989, 55:16771683.
［25］ Liao C H. Sullivan J, Grady J, et al. Biochemical characterization of pectate lyases produced by fluorescent Pseudomonads associated with spoilage of fresh fruits and vegetables. J Appl Microbiol,1997,83:1016.
［26］ David W Bauer, Alan Collmer. Molecular cloning, characterization, and mutagenesis of a pel gene from Pseudomonas syringae pv. lachrymans encoding a member of the Erwinia chrysanthemi PelADE family of pectate lyases M. The American Phytopathological Society,1997, 10:369379.
［27］ Stutzenberger F J. Inducible thermoalkalophilic polygalacturonate lyase from Thermomonospora fusca. J Bacteriol,1987,169:27742780.
［28］ Stephen G Walker, Maria E. Cloning and expression of a pectate lyase from the oral spirochete Treponema pectinovorum ATCC 33768. FEMS Microbiology Letters, 2003, 226:385390.
［29］ Judical Parisot, Ghochikyan A, Langlois V, et al. Exopolygalacturonate lyase from Thermotoga maritima: cloning, characterization and organic synthesis application.Carbohydrate Research,2002,337: 14271433.
［30］张健红,李寅,刘和,等.一株碱性果胶酶高产细菌的分离、系统发育分析和产酶条件的初步优化.应用与环境生物学报.2005,11(3):354358. Zhang J H,Li Y,Liu He, et al. Chin J Appl Environ Biol, 2005,11(3):354358.
［31］ Sridevi Annapurna Singh, Hans Plattner, Hans Diekmann. Exopolygalacturonate lyase from a thermophilic Bacillus sp. Enzyme and Microbial Technology, 1999,25: 420425.
［32］ Rao M N,Kembhavi A A,Pant A.Role of lysine,tryptophan and calcium in the βelimination activity of a lowmolecular mass pectate lyase from Fusarium moniliformae.Biochem J,1996,319:159164.
［33］汤克明,王强.芽孢杆菌产碱性果胶酶的棉散纤维精炼.印染,2009,9:1416. Tang K M,Wang Q.Dyeing and Finishing, 2009,9:1416.
［34］刘佩,李群.碱性果胶酶KDNT01及其复合酶在毛巾精练中的应用.纺织高校基础科学学报.2008,21(2):246248. Liu P, Li Q. Basic Sciences Journal of Textile Universities, 2008,21(2):246248.
［35］王槐传,叶汉玲,王清.优良碱性果胶酶产生菌选育.南京林业大学学报,1994,18(4):5154. Wang H Z,Ye H L,Wang Q.Journal of Nanjing Forestry University,1994,18(4):5154.
［36］李祖明,李鸿玉.碱性果胶酶诱导黄瓜抗病机理的初步研究.植物保护,2008,34(5):5257. Li Z M,Li H Y. Plant Protection, 2008，34(5):5257.
［37］李祖明, 李鸿玉.碱性果胶酶诱导植物抗病的研究.中国植物保护.2008,28(9):58. Li Z M,Li H Y. China Plant Protection,2008,28(9):58.