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中国生物工程杂志

CHINA BIOTECHNOLOGY
中国生物工程杂志  2010, Vol. 30 Issue (04): 120-124    
综述     
转录介导的扩增技术及其在诊断中的应用
高闪电,常惠芸**,独军政,丛国正,邵军军,林彤
中国农业科学院兰州兽医研究所 国家口蹄疫参考实验室 家畜疫病病原生物学国家重点实验室 农业部畜禽病毒学重点开放实验室 兰州 730046
Transcription Mediated Amplification and Its Applications in Diagnosis
GAO Shan-dian,CHANG Hui-yun,DU Jun-zheng,CONG Guo-zheng,SHAO Jun-jun,LIN Tong
Key Laboratory of Animal Virology of Ministry of Agriculture, State Key Laboratory of Veterinary Etiological Biology, National FMD Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046,China
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摘要:

转录介导的扩增技术可以RNA或DNA为模板,利用RNA聚合酶和逆转录酶在约42°C等温反应条件下进行扩增,产物为RNA。该技术在每一循环可产生模板的100~1000个拷贝转录本,15~30 min可将模板扩增约1010倍,在人类白细胞抗原等位基因分型、细菌和病毒的快速检测中发挥了重要作用。

关键词: 转录介导的扩增技术恒温扩增诊断    
Abstract:

Transcription Mediated Amplification is a target nucleic acid amplification method that uses RNA polymerase and reverse transcriptase to produce RNA amplicon from a target RNA or DNA under isothermal condition at about 42℃. TMA produces 100~1000 copies per cycle which results in a 10 billion fold increase of copies within about 15~30 minutes. TMA is now widely used in genotyping of human leukocyte antigen and diagnosis of many bacterium and viruses.

Key words: Transcription mediated amplification    Isothermal amplification    Diagnosis
收稿日期: 2009-06-25 出版日期: 2010-04-29
基金资助:

国家“十一五”重大科技支撑计划资助项目(2006BAD06A14,2006BAD06A17)

通讯作者: 常惠芸     E-mail: changhuiyun@126.com
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引用本文:

高闪电 常惠芸 独军政 丛国正 邵军军 林彤. 转录介导的扩增技术及其在诊断中的应用[J]. 中国生物工程杂志, 2010, 30(04): 120-124.

GAO Shan-Dian, CHANG Hui-Yun, DU Jun-Zheng, CONG Guo-Zheng, SHAO Jun-Jun, LIN Tong. Transcription Mediated Amplification and Its Applications in Diagnosis. China Biotechnology, 2010, 30(04): 120-124.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/        https://manu60.magtech.com.cn/biotech/CN/Y2010/V30/I04/120

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