技术与方法 |
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一种基于温敏质粒的新型基因敲除方法 |
姜娜1,2,王艳春2,马志宏1,罗琳1*,刘纯杰2* |
1.北京市农林科学院北京市水产科学研究所 北京 100068
2.军事医学科学院生物工程研究所 北京 100071 |
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A Novel Temperatrue Sensitive Plasmid-based Method for Deletion of Chromosomal Genes |
1.Beijing Fisheries Research Institute, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100068, China
2.Beijing Institute of Biotechnology, Beijing 100071, China |
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[5] 姜娜, 王芃, 王艳春, 等. 利用λRed重组系统敲除伤寒沙门氏菌rfaH基因. 生物技术通讯, 2009, 20(2): 174177. Jiang N, Wang P, Wang Y C, et al.Lett Biotechnol, 2009, 20(2): 174177.
[6] Hindle Z, Chatfield S N, Phillimore J, et al. Characterization of Salmonella enterica derivatives harboring defined aroC and Salmonella pathogenicity island 2 type III secretion system (ssaV) mutations by immunization of healthy volunteers. Infect Immun, 2002, 70(7): 34573467.
[7] Anne D, Biliana L, Denis D, et al. A rapid and simple method for inactivating chromosomal genes in Yersinia. FEMS Immunol Med Mic, 2003, 38:113116.
[8] 王恒梁, 冯尔玲, 史兆兴, 等. 用Red系统快速敲除痢疾杆菌asd基因. 军事医学科学院院刊, 2002, 26(3): 172175 Wang H L, Feng E L, Shi Z X,Bull Acad Mil Med Sci, 2002, 26(3): 172175. |
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