研究报告 |
|
|
|
|
BALB/C鼠冷诱导RNA结合蛋白的原核表达、纯化及其单克隆抗体的制备 |
李士泽1**,赵巧香1,辛九庆2,赵雅楠1,尹位1,杨焕民1,计红1 |
1.黑龙江八一农垦大学动物科技学院 大庆 163319
2.中国农业科学院哈尔滨兽医研究所 哈尔滨 150000 |
|
Prokaryotic Expression,Purification and Preparation of Monoclonal Antibodies of Cold Inducible RNA-binding Protein in BALB/C Mice |
1.College of Animal Science and Technology, Heilongjiang August First Land Reclamation University, Daqing 163319, China
2.Harbin Veterinary Research Insititue, Chinese Academy of Agricultural Sciences, Harbin 15000,China |
[1] Nishiyama H, Itoh K, Kaneko Y, et al. A glycinerich RNAbinding protein mediating coldinducible suppression of mammalian cell growth. J Cell Biol, 1997, 137(4):899908.
[2] van Venrooy S, Fichtner D, Kunz M. Coldinducible RNA binding protein (CIRP), a novel XTcf3 specific target gene regulates neural development in Xenopus. Bio Med Central, 2008,7(8):77.
[3] Peng Y, Kok K H, Xu R H, et al. Maternal cold inducible RNA binding protein is required for embryonic kidney formation in Xenopus laevis. FEBS Lett, 2000, 482(12)):3743.
[4] Tan H K,Yap M G. Overexpression of coldinducible RNAbinding protein increases interferongamma production in Chinesehamster ovary cells. Biotechnol Appl Biochem, 2008, 49(pt4):247257.
[5] Nishiyama H, Higashitsuji H, Yokoi H, et al. Cloning and characterization of human CIRP(coldinducible RNAbinding protein) cDNA and chromosomal assignment of the gene. Gene, 1997, 204(12):115120.
[6] Nishiyama H, Danno S, Kaneko Y, et al. Decreased expression of coldinducible RNAbinding protein (CIRP) in male germ cells at elevated temperature. Am J Pathol, 1998, 152(1):289296.
[7] Matsumoto K, Aoki K, Dohmae N, et al. CIRP2, a major cytoplasmic RNAbinding protein in Xenopus oocytes. Nucleic Acids Research, 2000, 28(23):46894697.
[8] Yoon S K, Hong J K, Choo S H, et al. Adaptation of Chinese hamster ovary cells to low culture temperature: Cell growth and recombinant protein production. J Biotechnol, 2006, 122(4): 463472.
[9] Nishiyama H, Xue J H, Sato T, et al. Diurnal change of the cold inducible RNAbinding protein (CIRP) expression in mouse brain. Biochem Biophys Res Commun, 1998, 245(2):534538.
[10] Saito T, Sugimoto K, Adachi Y, et al. Cloning and characterization of amphibian cold inducible RNAbinding protein. Com Biochem Physiol B Biochem Mol, 2000, 125(2):237245.
[11] Uochi T, Asashima M. XCIRP(Xenopus homolog of coldinducible RNAbinding protein) is expressed transiently in developing pronephros and neural tissue. Gene, 1998,211(2):245250.
[12] Leeuw F D, Zhang T, Wauquier C, et al. The coldinducible RNAbinding protein migrates from the nucleus to cytoplasmic stress granules by a methylationdependent mechanism and acts as a translational repressor. Exp Cell Res, 2007, 313(20):41304144.
[13] Tanaka K, Kawamura H, Matsugu H. An ascidian glycinerich RNA binding protein is not inducibled by temperature stress but is expressed under a genetic program during embryogenesis. Gene, 2000, 243(12):207214.
[14] Suqimoto K, Jiang H. Cold stress and light signals induce the expression of coldinducible RNA binding protein (cirp) in the brain and eye of the Japanese tree frog. Comp Biochem Physiol A Mol Inteq Physiol, 2008,151(4):324331.
[15] Hamid A A, Mandai M, Fujita J, et al. Expression of coldInducible RNAbinding protein in the normal endometrium, endometrial hyperplasia, and endometrial carcinoma. J Gynecol Pathol, 2003, 22(3):240247.
[16] 李士泽,金福厚,赵巧香,等.冷诱导RNA结合蛋白的生物学功能研究进展.生理科学进展,2009,40(3):271273. Li S Z,Jin F H,Zhao Q X,et al.Chinese Journal of Applied & Environmental Biology,2009,15(1):8790.
[17] 金福厚,庞岩,李士泽,等.BALB/C鼠睾丸组织中冷诱导RNA结合蛋白的cDNA克隆与序列分析.应用与环境生物学报,2009,15(1):8790. Jin F H,Pang Y,Li S Z,et al.Progress in Physiological Sciences,2009,40(3):271273. |
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
|
Shared |
|
|
|
|
|
Discussed |
|
|
|
|