巴西橡胶树转基因研究现状与展望

中国生物工程杂志

2010, Vol. 30

Issue (01): 85-92

综述

巴西橡胶树转基因研究现状与展望

邹智^**,杨礼富,王真辉,袁坤

中国热带农业科学院橡胶研究所农业部橡胶树生物学重点开放实验室儋州 571737

Advances and Perspectives on Genetic Modification of Hevea brasiliensis

ZOU Zhi,YANG Li-fu,WANG Zhen-hui,YUAN Kun

Rubber Research Institute, Chinese Academy of Tropical Agricultural Sciences, Key Laboratory of Rubber Biology,Ministry of Agriculture, Danzhou 57173,China

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摘要：

由于存在遗传背景狭窄、高度杂合化、育种周期长等特点，巴西橡胶树育种进展非常缓慢。转基因技术为拓展其的遗传范围、加速育种进程提供了契机。在过去20年里，橡胶树转基因研究取得了很大进展：成功应用的转化技术包括基因枪法和农杆菌介导法，受体材料包括花药和内珠被愈伤，育种目标涉及到提高胶乳产量、抗死皮和作为生物反应器等。但同时也存在组培程序不够成熟、转化技术和外植体比较单一、转化效率低下等问题。最后，对以后转化体系的优化、转基因育种方向进行了分析和展望。

关键词： 橡胶树; 遗传转化; 农杆菌介导法; 基因枪转化法

Abstract:

As the major commercial source of natural rubber, Hevea brasiliensis attracts much attention. However, the heterozygous nature, long breeding cycle are strong limitations for conventional breeding. While genetic engineering, which can be used to widen the germplasm base and produce desirable agronomic traits quickly and efficiently, offers a viable alternative approach to complement traditional breeding. Comprehensive analysis indicates that in the past two decades, with calli derived from immature anther or integumental tissues of immature fruit as receptors, both biolistic and Agrobacterium-mediated transformation methods were employed for developping rubber genotypes with improved latex yield, tolerance to tapping panel dryness syndrome, producing high-value recombinant proteins, etc. Being recalcitrant to tissue culture, the transformation efficiency of Hevea is comparatively low, and the procedures are still needed to optimize. Finally, breeding objectives and strategies to improve transformation efficiency were also proposed in the review.

Key words: Hevea brasiliensis genetic modification Agrobacterium-mediated transformation Biolistic transformation

收稿日期: 2009-08-28 出版日期: 2010-01-27

基金资助:

中央级公益性科研院所基本科研业务费专项［XJSYWFZX200912、YWFZX0904(N)］、公益性行业科技专项经费(nyhyzx070331)资助项目

通讯作者: 邹智 E-mail: zouzhi2008@126.com

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引用本文:

邹智杨礼富王真辉袁坤. 巴西橡胶树转基因研究现状与展望[J]. 中国生物工程杂志, 2010, 30(01): 85-92.

JU Zhi, YANG Li-Fu, WANG Zhen-Hui, YUAN Kun. Advances and Perspectives on Genetic Modification of Hevea brasiliensis. China Biotechnology, 2010, 30(01): 85-92.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/ 或 https://manu60.magtech.com.cn/biotech/CN/Y2010/V30/I01/85

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