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中国生物工程杂志

CHINA BIOTECHNOLOGY
中国生物工程杂志
中国生物工程杂志  2010, Vol. 30 Issue (01): 62-66    
研究报告     
流加发酵对重组Bacillus subtilis发酵生产角质酶的影响
陈晟1,2,张芙华1,2,陈坚1,2,吴敬1,2**
1.江南大学生物工程学院 工业生物技术教育部重点实验室 无锡 214122
2.江南大学食品科学与技术国家重点实验室 无锡 214122
Effects of Fed-fermentation on Cutinase Production by Recombinant Bacillus subtilis
CHEN Sheng1,2,ZHANG Fu-hua1,2,CHEN Jian1,2,WU Jing1,2
1.School of Biotechnology and Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China
2.State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China
 全文: PDF(702 KB)   HTML
摘要：

为实现基因工程菌Bacillus subtilis WSHB06-07生产角质酶的高产，在3L发酵罐中考察了不同初糖浓度对菌体生长和产酶的影响，并在选择38 g/L初始蔗糖浓度的基础上，进行碳源的分批流加和恒速流加，结果表明发酵16 h开始流加碳源，采用总补糖量60g/L，蔗糖平均流速为4g/(L·h)的恒速补料方式，角质酶酶活在31h可达到最大545.87U/ml，比分批发酵酶活提高67.8%，并获得较高的角质酶生产强度，满足工业化生产要求。
关键词： Bacillus subtilis;  重组角质酶;  分批流加发酵;  连续流加发酵;  碳源;  生产强度    
Abstract:

In order to achieve the high production of cutinase by recombinant Bacillus subtilis WSHB06-07, the effect of various initial sucrose concentration on enzyme production was investigated in 3 L fermentor. It was found that sucrose concentration of 38 g/L is suitable for cell growth and cutinase production. Based on this sucrose concentration, fedbatch as well as constant-fed fermentation were investigated. The results showed that, using constant-fed fermentation from 16 h to 31 h and sucrose feed rate of 4 g/(L·h), the cutinase activity in the culture media reached 545.87U/ml, which was increased by 67.8% compared to that of batch fermentation. By this way, high cutinase productivity was gained and it is beneficial for industrial production.
Key words: Bacillus subtilis    recombinant cutinase    fed-batch fermentation    constant-fed fermentation    carbon source    cutinase productivity
收稿日期: 2009-09-01 出版日期: 2010-01-27
基金资助:

国家“863”计划(2009AA02Z204)、教育部新世纪优秀人才支持计划(NCET-06-0486)、食品科学与技术国家重点实验室科研基金(SKLF-MB-200802)资助项目
通讯作者: 吴敬     E-mail: jingwu@jiangnan.edu.cn
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引用本文:

陈晟 张芙华 陈坚 吴敬. 流加发酵对重组Bacillus subtilis发酵生产角质酶的影响[J]. 中国生物工程杂志, 2010, 30(01): 62-66.

CHEN Cheng, ZHANG Fu-Hua, CHEN Jian, TUN Jing. Effects of Fed-fermentation on Cutinase Production by Recombinant Bacillus subtilis. China Biotechnology, 2010, 30(01): 62-66.

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https://manu60.magtech.com.cn/biotech/CN/    或    https://manu60.magtech.com.cn/biotech/CN/Y2010/V30/I01/62

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