技术与方法 |
|
|
|
|
反向斑点杂交法快速检测HBV基因型反应条件的优化和建立* |
赵丽,张文露,胡源,刘彦辰,赖国旗,杨凤,黄爱龙**
|
重庆医科大学感染性疾病分子生物学教育部重点实验室 重庆 400016 |
|
Development of a Reverse Dot Blot System for rapid detection of HBV Genotypes |
ZHAO Li,ZHANG Wen-lu,HU Yuan,LIU Yan-chen,LAI Guo-qi,YANG Feng,HUANG Ai-long |
Key Laboratory of Molecular Biology on Infectious Diseases,Ministry of Education, Chongqing Medical University ,Chongqing 400016,China |
引用本文:
赵丽 张文露 胡源 刘彦辰 赖国旗 杨凤 黄爱龙. 反向斑点杂交法快速检测HBV基因型反应条件的优化和建立*[J]. 中国生物工程杂志, 2009, 29(12): 85-89.
DIAO Li, ZHANG Wen-Lou, HU Yuan, LIU Pan-Chen, LAI Guo-Qi, YANG Feng, HUANG Ai-Long. Development of a Reverse Dot Blot System for rapid detection of HBV Genotypes. China Biotechnology, 2009, 29(12): 85-89.
链接本文:
https://manu60.magtech.com.cn/biotech/CN/
或
https://manu60.magtech.com.cn/biotech/CN/Y2009/V29/I12/85
|
[1] |
McMahon B J. Epidemiology and natural history of hepatitis B. Semin Liver Dis,2005,25(Suppl)1:3~8
|
[2] |
Okamoto H F,Tsuda H,Sakugawa R I,et al. Typing hepatitis B virus by homology in nucleotide sequence: comparison of surface antigen subtypes. J Gen Virol,1988,69:2575~2583
|
[3] |
Norder H, Courouce A M, Magnius L O. Complete genomes, phylogenetic relatedness, and structural proteins of six strains of the hepatitis B virus, four of which represent two new genotypes. Virology ,1994,198:489~503
|
[4] |
Zeng G,Wang Z,Wen S,et a1.Geographic distribution,virologic and clinical characteristics of hepatitis B virus genotypes in China.J Viral Hepat,2005,12:609~617
|
[5] |
Thakur V, Guptan R C, Kazim S N, et al. Profile, spectrum and significance of HBV genotypes in chronic liver disease patients in the Indian subcontinent. J Gastroenterol Hepatol ,2002,17(2):165~170
|
[6] |
Lee C M,Chen C H,Lu S N,et al. Prevalence and clinical implications of hepatitis B virus genotypes in southern Taiwan. Scand J Gastroenterol,2003,38(1):95-101
|
[7] |
Wai C T,Chu C J,Hussain M,et al. HBV genotype B is associated with better response to interferon therapy in HBeAg(+)chronic hepatitis than genotype C. Hepatology ,2002,36(6):1425~1430
|
[8] |
Saiki R K,Walsh P S,Levenson C H,et al. Genetic analysis of amplified DNA with immobilized sequencespecific oligonucleotide probes. Proc Natl Acad Sci USA,1989,86:6230~6234
|
[9] |
Ou Z Y, Liu N, Chen C J,et al. Rapid and accurate genotyping of YMDD motif variants in the hepatitis B virus genome by an improved reverse dot blot method. J Clin Microbiol, 2005, 43(11): 5685~5689
|
[10] |
Lovicu M,Dessi V,Zappu A ,et al. Efficient strategy for molecular diagnosis of Wilson Disease in the Sardinian population. Clinical Chemistry, 2003, 49(3):496~498
|
[11] |
Sambrook J, Fritsch E F, Maniatis T,et al. Molecular Cloning: A laboratory Manual.2nd ed. New York:Cold Spring Harbor Laboratory Press, 1989
|
[12] |
Lindh M,Andersson A S,Gusdal A,et a1.Genotypes,nt1858 variants,and geographic origin of hepatitis B virus large scale analysis using a new genotyping method.J Infectious Disease,1997,175:1285~1293
|
[13] |
Naito H, Hayashi S,Abe K. Rapid and specific genotyping system for hepatitis B virus corresponding to six major genotypes by PCR using typespecific primers. J Clin Microbiol,2001, 39:362~364
|
[14] |
Usuda S,Okamoto H,Tanaka T,et a1.Differentiation of hepatitis B virus genotypes D and E by ELISA using monoclonal antibodies to epitopes on the preS2region product. J Virol Methods, 2000, 87:81~89
|
[15] |
Norder H, Hammas B, Lofdahl S,et al. Comparison of the amino acid sequences of nine different serotypes of hepatitis B surface antigen and genomic classification of the corresponding hepatitis B virus strains. J Gen Virol,1992,73: 1201~1208
|
[16] |
肖蕾,王雪刚,聂军等.中国广东地区乙型肝炎病毒基因亚型的分布.肝脏.2006,6(11):149~151
|
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
|
Shared |
|
|
|
|
|
Discussed |
|
|
|
|