响应面设计法优化蕨渣基质的灵芝培养条件

中国生物工程杂志

2009, Vol. 29

Issue (12): 37-42

研究报告

响应面设计法优化蕨渣基质的灵芝培养条件

余有贵^1,2*,李忠海¹,黄国华²,陈彩琼²

1.中南林业科技大学长沙 410004,
2.邵阳学院邵阳 422000

Optimization of Cultural Condition of Ganoderma lucidum in the Compound Culture Medium by Response Surface Methodology

YU You-gui^1,2,LI Zhong-hai¹,HUANG Guo-hua²,CHEN Cai-qiong²

1.Central South University of Forestry Technology，Changsha 410004
2.Shaoyang University,Shaoyang 422000，China

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摘要：

探索灵芝在以蕨渣为主要成分的固态基质中的培养条件，可为蕨渣的开发利用提供理论依据。以蕨渣为主要原料，采用响应面法对灵芝培养条件（基质蕨渣比例、基质含水量和培养温度）进行优化。结果表明，基质蕨渣比例、基质含水量和培养温度对灵芝菌丝日平均生长速率均有极显著的影响（p<0.01），且基质含水量与培养温度之间、基质蕨渣比例与基质含水量之间存在交互作用。优化出灵芝培养条件为蕨渣比例85%，基质含水量62.5%，培养温度27℃，在此条件下,灵芝菌丝日平均生长速率为3.48mm/d。多元回归分析结果显示，基质蕨渣比例、基质含水量、培养温度与菌丝日平均生长速率之间回归模型高度显著，可用于实际生产预测。首次报道了利用蕨渣培养灵芝,为蕨渣进一步的开发研究奠定基础。

关键词： 蕨渣; 灵芝; 培养条件; 生长速率; 响应面法

Abstract:

The purpose is to obtain optimal cultural conditions for Ganoderma lucidum in the solid medium mainly containing the residual of Pteridium aquilinum,and to provide theoretical foundation for the further use of this material from Pteridium aquilinum.Response surface methodology was applied to optimize the main culture conditions including proportion of the residual compound in medium,water content in medium and culture temperature.The results showed that proportion of residual of Pteridium aquilinum in medium,water content in medium and cultural condition had noticeably significant effects on daily average growth rate of mycelium of Ganoderma lucidum(p<0.01).Obviously correlation was found to be existed between proportion of residual of Pteridium aquilinum and water content in medium,or water content in medium and the cultural temperature.The optimal proportion of residual of Pteridium aquilinum in medium was 85.0%.The optimal water content in medium was 62.4%,and the optimal cultural temperature was 27℃.Under these optimized conditions,daily average growth rate of mycelium of Ganoderma lucidum was 3.48mm/d.Multivariate regression analysis showed that the model for regressing proportion of residual of Pteridium aquilinum in medium,water content in medium,cultural temperature and daily average growth rate of mycelium of Ganoderma lucidum might be available for the culture of the fungi.Practical growth rate of mycelium of Ganoderma lucidum could be forecasted by applying the model. The application of residual of Pteridium aquilinum as a main additive in the culture of Ganoderma lucidum was firstly reported.It has laid a pave for the further investigation of residual of Pteridium aquilinum in medium.

Key words: Residual of Pteridium aquilinum Ganoderma lucidum Cultural condition Growth rate Response surface methodology

收稿日期: 2009-07-07 出版日期: 2009-12-21

ZTFLH:

Q93-335

通讯作者: 余有贵 E-mail: yufly225@yahoo.com.cn

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引用本文:

余有贵李忠海黄国华陈彩琼. 响应面设计法优化蕨渣基质的灵芝培养条件[J]. 中国生物工程杂志, 2009, 29(12): 37-42.

TU Wei-Gui, LI Zhong-Hai, HUANG Guo-Hua, CHEN Cai-Qiong. Optimization of Cultural Condition of Ganoderma lucidum in the Compound Culture Medium by Response Surface Methodology. China Biotechnology, 2009, 29(12): 37-42.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/ 或 https://manu60.magtech.com.cn/biotech/CN/Y2009/V29/I12/37

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